The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 11 Apr 2006
doi: 10.1242/jcs.02903
Research Article
Dynamic microtubules regulate the local concentration of E-cadherin at cell-cell contacts
Samantha J. Stehbens,
Andrew D. Paterson,
Matthew S. Crampton,
Annette M. Shewan,
Charles Ferguson,
Anna Akhmanova,
Robert G. Parton,
and
Alpha S. Yap*
* Author for correspondence (e-mail: a.yap{at}imb.uq.edu.au)
In contrast to the well-established relationship between cadherins and the actin cytoskeleton, the potential link between cadherins and microtubules (MTs) has been less extensively investigated. We now identify a pool of MTs that extend radially into cell-cell contacts and are inhibited by manoeuvres that block the dynamic activity of MT plus-ends (e.g. in the presence of low concentrations of nocodazole and following expression of a CLIP-170 mutant). Blocking dynamic MTs perturbed the ability of cells to concentrate and accumulate E-cadherin at cell-cell contacts, as assessed both by quantitative immunofluorescence microscopy and fluorescence recovery after photobleaching (FRAP) analysis, but did not affect either transport of E-cadherin to the plasma membrane or the amount of E-cadherin expressed at the cell surface. This indicated that dynamic MTs allow cells to concentrate E-cadherin at cell-cell contacts by regulating the regional distribution of E-cadherin once it reaches the cell surface. Importantly, dynamic MTs were necessary for myosin II to accumulate and be activated at cadherin adhesive contacts, a mechanism that supports the focal accumulation of E-cadherin. We propose that this population of MTs represents a novel form of cadherin-MT cooperation, where cadherin adhesions recruit dynamic MTs that, in turn, support the local concentration of cadherin molecules by regulating myosin II activity at cell-cell contacts.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
W.-M. Heupel, A. Efthymiadis, N. Schlegel, T. Muller, Y. Baumer, W. Baumgartner, D. Drenckhahn, and J. Waschke
Endothelial barrier stabilization by a cyclic tandem peptide targeting VE-cadherin transinteraction in vitro and in vivo
J. Cell Sci.,
May 15, 2009;
122(10):
1616 - 1625.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. D. Mruk, B. Silvestrini, and C. Y. Cheng
Anchoring Junctions As Drug Targets: Role in Contraceptive Development
Pharmacol. Rev.,
June 1, 2008;
60(2):
146 - 180.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Liu, S. Woolner, J. E. Johndrow, D. Metzger, A. Flores, and S. M. Parkhurst
Sisyphus, the Drosophila myosin XV homolog, traffics within filopodia transporting key sensory and adhesion cargos
Development,
January 1, 2008;
135(1):
53 - 63.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Carramusa, C. Ballestrem, Y. Zilberman, and A. D. Bershadsky
Mammalian diaphanous-related formin Dia1 controls the organization of E-cadherin-mediated cell-cell junctions
J. Cell Sci.,
November 1, 2007;
120(21):
3870 - 3882.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. W. McLachlan, A. Kraemer, F. M. Helwani, E. M. Kovacs, and A. S. Yap
E-Cadherin Adhesion Activates c-Src Signaling at Cell Cell Contacts
Mol. Biol. Cell,
August 1, 2007;
18(8):
3214 - 3223.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. P. Maddugoda, M. S. Crampton, A. M. Shewan, and A. S. Yap
Myosin VI and vinculin cooperate during the morphogenesis of cadherin cell cell contacts in mammalian epithelial cells
J. Cell Biol.,
July 24, 2007;
178(3):
529 - 540.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. N. Nejsum and W. J. Nelson
A molecular mechanism directly linking E-cadherin adhesion to initiation of epithelial cell surface polarity
J. Cell Biol.,
July 10, 2007;
178(2):
323 - 335.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Siegrist and C. Q. Doe
Microtubule-induced cortical cell polarity
Genes & Dev.,
March 1, 2007;
21(5):
483 - 496.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2006
