spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 23 May 2006
doi: 10.1242/jcs.02970

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.02970v1
119/12/2445    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McQuade, K. J.
Right arrow Articles by Rapraeger, A. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McQuade, K. J.
Right arrow Articles by Rapraeger, A. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Syndecan-1 regulates {alpha}v{beta}5 integrin activity in B82L fibroblasts


Kyle J. McQuade, DeannaLee M. Beauvais, Brandon J. Burbach, and Alan C. Rapraeger*
* Author for correspondence (e-mail: acraprae{at}wisc.edu)

B82L mouse fibroblasts respond to fibronectin or vitronectin via a syndecan-1-mediated activation of the {alpha}v{beta}5 integrin. Cells attached to syndecan-1-specific antibody display only filopodial extension. However, the syndecan-anchored cells extend lamellipodia when the antibody-substratum is supplemented with serum, or low concentrations of adsorbed vitronectin or fibronectin, that are not sufficient to activate the integrin when plated alone. Integrin activation is blocked by treatment with (Arg-Gly-Asp)-containing peptides and function-blocking antibodies that target {alpha}v integrins, as well as by siRNA-mediated silencing of {beta}5 integrin expression. In addition, {alpha}v{beta}5-mediated cell attachment and spreading on high concentrations of vitronectin is blocked by competition with recombinant syndecan-1 ectodomain core protein and by downregulation of mouse syndecan-1 expression by mouse-specific siRNA. Taking advantage of the species-specificity of the siRNA, rescue experiments in which human syndecan-1 constructs are expressed trace the activation site to the syndecan-1 ectodomain. Moreover, both full-length mouse and human syndecan-1 co-immunoprecipitate with the {beta}5 integrin subunit, but fail to do so if the syndecan is displaced by competition with soluble, recombinant syndecan-1 ectodomain. These results suggest that the ectodomain of the syndecan-1 core protein contains an active site that assembles into a complex with the {alpha}v{beta}5 integrin and regulates {alpha}v{beta}5 integrin activity.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
A. M. Avalos, A. D. Valdivia, N. Munoz, R. Herrera-Molina, J. C. Tapia, S. Lavandero, M. Chiong, K. Burridge, P. Schneider, A. F. G. Quest, et al.
Neuronal Thy-1 induces astrocyte adhesion by engaging syndecan-4 in a cooperative interaction with {alpha}v{beta}3 integrin that activates PKC{alpha} and RhoA
J. Cell Sci., October 1, 2009; 122(19): 3462 - 3471.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
E. Araki, Y. Momota, T. Togo, M. Tanioka, K. Hozumi, M. Nomizu, Y. Miyachi, and A. Utani
Clustering of Syndecan-4 and Integrin {beta}1 by Laminin {alpha}3 Chain-derived Peptide Promotes Keratinocyte Migration
Mol. Biol. Cell, July 1, 2009; 20(13): 3012 - 3024.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
D. M. Beauvais, B. J. Ell, A. R. McWhorter, and A. C. Rapraeger
Syndecan-1 regulates {alpha}v{beta}3 and {alpha}v{beta}5 integrin activation during angiogenesis and is blocked by synstatin, a novel peptide inhibitor
J. Exp. Med., March 16, 2009; 206(3): 691 - 705.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. R. Kliment, J. M. Englert, B. R. Gochuico, G. Yu, N. Kaminski, I. Rosas, and T. D. Oury
Oxidative Stress Alters Syndecan-1 Distribution in Lungs with Pulmonary Fibrosis
J. Biol. Chem., February 6, 2009; 284(6): 3537 - 3545.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
M. Murakami, A. Elfenbein, and M. Simons
Non-canonical fibroblast growth factor signalling in angiogenesis
Cardiovasc Res, May 1, 2008; 78(2): 223 - 231.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. Gotte, D. Spillmann, G. W. Yip, E. Versteeg, F. G. Echtermeyer, T. H. van Kuppevelt, and L. Kiesel
Changes in heparan sulfate are associated with delayed wound repair, altered cell migration, adhesion and contractility in the galactosyltransferase I (ss4GalT-7) deficient form of Ehlers-Danlos syndrome
Hum. Mol. Genet., April 1, 2008; 17(7): 996 - 1009.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y. Yang, V. MacLeod, Y. Dai, Y. Khotskaya-Sample, Z. Shriver, G. Venkataraman, R. Sasisekharan, A. Naggi, G. Torri, B. Casu, et al.
The syndecan-1 heparan sulfate proteoglycan is a viable target for myeloma therapy
Blood, September 15, 2007; 110(6): 2041 - 2048.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. A. Stepp, Y. Liu, S. Pal-Ghosh, R. A. Jurjus, G. Tadvalkar, A. Sekaran, K. LoSicco, L. Jiang, M. Larsen, L. Li, et al.
Reduced migration, altered matrix and enhanced TGFbeta1 signaling are signatures of mouse keratinocytes lacking Sdc1
J. Cell Sci., August 15, 2007; 120(16): 2851 - 2863.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. A. Kirkpatrick and S. B. Selleck
Heparan sulfate proteoglycans at a glance
J. Cell Sci., June 1, 2007; 120(11): 1829 - 1832.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Su, S. A. Blaine, D. Qiao, and A. Friedl
Shedding of Syndecan-1 by Stromal Fibroblasts Stimulates Human Breast Cancer Cell Proliferation via FGF2 Activation
J. Biol. Chem., May 18, 2007; 282(20): 14906 - 14915.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
P. Ma, S. L. Beck, R. W. Raab, R. L. McKown, G. L. Coffman, A. Utani, W. J. Chirico, A. C. Rapraeger, and G. W. Laurie
Heparanase deglycanation of syndecan-1 is required for binding of the epithelial-restricted prosecretory mitogen lacritin
J. Cell Biol., September 25, 2006; 174(7): 1097 - 1106.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006