spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 1 Aug 2006
doi: 10.1242/jcs.03078

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.03078v1
119/16/3443    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Branche, C.
Right arrow Articles by Bastin, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Branche, C.
Right arrow Articles by Bastin, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Conserved and specific functions of axoneme components in trypanosome motility


Carole Branche, Linda Kohl, Géraldine Toutirais, Johanna Buisson, Jacky Cosson, and Philippe Bastin*
* Author for correspondence (e-mail: pbastin{at}pasteur.fr)

The Trypanosoma brucei flagellum is unusual as it is attached along the cell body and contains, in addition to an apparently conventional axoneme, a structure called the paraflagellar rod, which is essential for cell motility. Here, we investigated flagellum behaviour in normal and mutant trypanosome cell lines where expression of genes encoding various axoneme proteins (PF16, PF20, DNAI1, LC2) had been silenced by RNAi. First, we show that the propulsive wave (normally used for forward motility) is abolished in the absence of outer dynein arms, whereas the reverse wave (normally used for changing direction) still occurs. Second, in contrast to Chlamydomonas - but like metazoa, the central pair adopts a fixed orientation during flagellum beating. This orientation becomes highly variable in central-pair- and outer-dynein-arm-mutants. Third, the paraflagellar rod contributes to motility by facilitating three-dimensional wave propagation and controlling cell shape. Fourth, motility is required to complete the last stage of cell division in both insect and bloodstream stages of the parasite. Finally, our study also reveals the conservation of molecular components of the trypanosome flagellum. Coupled to the ease of reverse genetics, it raises the interest of trypanosomes as model organisms to study cilia and flagella.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 JCBHome page
R. S. Patel-King and S. M. King
An outer arm dynein light chain acts in a conformational switch for flagellar motility
J. Cell Biol., July 27, 2009; 186(2): 283 - 295.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. Adhiambo, T. Blisnick, G. Toutirais, E. Delannoy, and P. Bastin
A novel function for the atypical small G protein Rab-like 5 in the assembly of the trypanosome flagellum
J. Cell Sci., March 15, 2009; 122(6): 834 - 841.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Portman, S. Lacomble, B. Thomas, P. G. McKean, and K. Gull
Combining RNA Interference Mutants and Comparative Proteomics to Identify Protein Components and Dependences in a Eukaryotic Flagellum
J. Biol. Chem., February 27, 2009; 284(9): 5610 - 5619.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Absalon, T. Blisnick, M. Bonhivers, L. Kohl, N. Cayet, G. Toutirais, J. Buisson, D. Robinson, and P. Bastin
Flagellum elongation is required for correct structure, orientation and function of the flagellar pocket in Trypanosoma brucei
J. Cell Sci., November 15, 2008; 121(22): 3704 - 3716.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
Z. Li and C. C. Wang
KMP-11, a Basal Body and Flagellar Protein, Is Required for Cell Division in Trypanosoma brucei
Eukaryot. Cell, November 1, 2008; 7(11): 1941 - 1950.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Absalon, T. Blisnick, L. Kohl, G. Toutirais, G. Dore, D. Julkowska, A. Tavenet, and P. Bastin
Intraflagellar Transport and Functional Analysis of Genes Required for Flagellum Formation in Trypanosomes
Mol. Biol. Cell, March 1, 2008; 19(3): 929 - 944.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. Griffiths, N. Portman, P. R. Taylor, S. Gordon, M. L. Ginger, and K. Gull
RNA Interference Mutant Induction In Vivo Demonstrates the Essential Nature of Trypanosome Flagellar Function during Mammalian Infection
Eukaryot. Cell, July 1, 2007; 6(7): 1248 - 1250.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. M. Baron, Z. P. Kabututu, and K. L. Hill
Stuck in reverse: loss of LC1 in Trypanosoma brucei disrupts outer dynein arms and leads to reverse flagellar beat and backward movement
J. Cell Sci., May 1, 2007; 120(9): 1513 - 1520.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. M. Baron, K. S. Ralston, Z. P. Kabututu, and K. L. Hill
Functional genomics in Trypanosoma brucei identifies evolutionarily conserved components of motile flagella
J. Cell Sci., February 1, 2007; 120(3): 478 - 491.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006