spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 5 Aug 2008
doi: 10.1242/jcs.031708

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.031708v1
121/17/2850    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hoogstraten, D.
Right arrow Articles by Houtsmuller, A. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hoogstraten, D.
Right arrow Articles by Houtsmuller, A. B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Versatile DNA damage detection by the global genome nucleotide excision repair protein XPC


Deborah Hoogstraten, Steven Bergink, Vincent H.M. Verbiest, Martijn S. Luijsterburg, Bart Geverts, Anja Raams, Christoffel Dinant, Jan H.J. Hoeijmakers, Wim Vermeulen*, and Adriaan B. Houtsmuller
* Author for correspondence (e-mail: w.vermeulen{at}erasmusmc.nl)

To investigate how the nucleotide excision repair initiator XPC locates DNA damage in mammalian cell nuclei we analyzed the dynamics of GFP-tagged XPC. Photobleaching experiments showed that XPC constantly associates with and dissociates from chromatin in the absence of DNA damage. DNA-damaging agents retard the mobility of XPC, and UV damage has the most pronounced effect on the mobility of XPC-GFP. XPC exhibited a surprising distinct dynamic behavior and subnuclear distribution compared with other NER factors. Moreover, we uncovered a novel regulatory mechanism for XPC. Under unchallenged conditions, XPC is continuously exported from and imported into the nucleus, which is impeded when NER lesions are present. XPC is omnipresent in the nucleus, allowing a quick response to genotoxic stress. To avoid excessive DNA probing by the low specificity of the protein, the steady-state level in the nucleus is controlled by nucleus-cytoplasm shuttling, allowing temporally higher concentrations of XPC in the nucleus under genotoxic stress conditions.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 JCBHome page
J. A. Marteijn, S. Bekker-Jensen, N. Mailand, H. Lans, P. Schwertman, A. M. Gourdin, N. P. Dantuma, J. Lukas, and W. Vermeulen
Nucleotide excision repair-induced H2A ubiquitination is dependent on MDC1 and RNF8 and reveals a universal DNA damage response
J. Cell Biol., September 21, 2009; 186(6): 835 - 847.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
C. Dinant, M. S. Luijsterburg, T. Hofer, G. von Bornstaedt, W. Vermeulen, A. B. Houtsmuller, and R. van Driel
Assembly of multiprotein complexes that control genome function
J. Cell Biol., April 6, 2009; 185(1): 21 - 26.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. Solimando, M. S. Luijsterburg, L. Vecchio, W. Vermeulen, R. van Driel, and S. Fakan
Spatial organization of nucleotide excision repair proteins after UV-induced DNA damage in the human cell nucleus
J. Cell Sci., January 1, 2009; 122(1): 83 - 91.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Alekseev, M. S. Luijsterburg, A. Pines, B. Geverts, P.-O. Mari, G. Giglia-Mari, H. Lans, A. B. Houtsmuller, L. H. F. Mullenders, J. H. J. Hoeijmakers, et al.
Cellular Concentrations of DDB2 Regulate Dynamic Binding of DDB1 at UV-Induced DNA Damage
Mol. Cell. Biol., December 15, 2008; 28(24): 7402 - 7413.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2008