spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 17 Oct 2006
doi: 10.1242/jcs.03202

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.03202v1
119/21/4510    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Calera, M. R.
Right arrow Articles by Goodenough, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Calera, M. R.
Right arrow Articles by Goodenough, D. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Connexin43 is required for production of the aqueous humor in the murine eye


Mónica R. Calera, Heather L. Topley, Yongbo Liao, Brian R. Duling, David L. Paul, and Daniel A. Goodenough*
* Author for correspondence (e-mail: dgoodenough{at}hms.harvard.edu)

Connexin43 is a major component of the gap junctions between pigmented and non-pigmented cells of the double-layered epithelium in the ciliary body of the eye. We directly tested the hypothesis that gap junctions play a crucial role in the production of the aqueous humor by inactivating the GJA1 (connexin43) gene in the pigmented epithelium with cre-loxP technology. To accomplish this, we crossed a line expressing cre recombinase driven by the nestin promoter and a line with floxed connexin43 alleles. Resultant lines exhibited loss of connexin43 from the pigmented epithelium, iris, retinal pigment epithelium and the lens. We observed plasma proteins in the aqueous humor and pathological changes consistent with a loss of intraocular pressure. As the ciliary body is responsible for aqueous humor production, these data support the hypothesis that the gap junctions between pigmented and non-pigmented epithelium are necessary for production of the aqueous humor that is in turn required for the generation of normal intraocular pressure and nourishment of the postnatal lens. The loss of connexin43 expression in the iris correlated with a separation of the posterior pigmented epithelium from the anterior myoepithelium and with meiosis, possibly resulting from a loss of function of the dilator pupillae.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Neurosci.Home page
J. S. Satz, A. R. Philp, H. Nguyen, H. Kusano, J. Lee, R. Turk, M. J. Riker, J. Hernandez, R. M. Weiss, M. G. Anderson, et al.
Visual Impairment in the Absence of Dystroglycan
J. Neurosci., October 21, 2009; 29(42): 13136 - 13146.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. R. Calera, Z. Wang, R. Sanchez-Olea, D. L. Paul, M. M. Civan, and D. A. Goodenough
Depression of Intraocular Pressure Following Inactivation of Connexin43 in the Nonpigmented Epithelium of the Ciliary Body
Invest. Ophthalmol. Vis. Sci., May 1, 2009; 50(5): 2185 - 2193.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
Y. Zhang, B. R. Davidson, W. D. Stamer, J. K. Barton, L. Y. Marmorstein, and A. D. Marmorstein
Enhanced Inflow and Outflow Rates Despite Lower IOP in Bestrophin-2-Deficient Mice
Invest. Ophthalmol. Vis. Sci., February 1, 2009; 50(2): 765 - 770.
[Abstract] [Full Text] [PDF]

Home page
 JDRHome page
A. Fenwick, R.J. Richardson, J. Butterworth, M.J. Barron, and M.J. Dixon
Novel Mutations in GJA1 Cause Oculodentodigital syndrome
Journal of Dental Research, November 1, 2008; 87(11): 1021 - 1026.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006