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Quarterly Journal of Microscopical Science, Vol s2-79, 73-90, Copyright © 1936 by Company of Biologists

Memoirs: The Effect of Ultra-Centrifuging Vertebrate Neurones

R. H. J. Brown B.A., B.Sc.1

1 School of Zoology, Trinity College, Dublin

1. The Golgi apparatus may appear as a network or incomplete reticulum; it is lighter than the other cytoplasmic inclusions but its form makes its displacement difficult. Its parts never approach the periphery of the cell. The neutral-red bodies have no part in its composition.

2. There exists a separate canalicular system which is connected with the surface of the cell, and otherwise is of similar dimensions to the Golgi apparatus. It is thought to represent the trophospongium of Holmgren. It is unaffected by the centrifuge.

3. The vacuome appears in the form of isolated granules which can be osmicated after staining in neutral red. They are lighter than the cytoplasm and are separate from the Golgi apparatus, though on account of their similar density they are thought to have some spatial connexion with it.

4. The mitochondria are in the form of rods and granules which are very slightly denser than the cytoplasm, and show no evidence of having any connexion with the Golgi apparatus.

5. The Nissl substance occurs as large irregular bodies in the fixed material. It is thought to be in a diffuse form in the living cell. It is much denser than the cytoplasm.







© The Company of Biologists Ltd 1936