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Quarterly Journal of Microscopical Science, Vol s3-100, 17-23, Copyright © 1959 by Company of Biologists

A Phase Contrast Study of the Blood-cells in Prodenia Larvae (Order Lepidoptera)

JACK COLVARD JONES 1

1 Department of Entomology, University of Maryland, College Park, Maryland, U.S.A.

The various classes of blood-cells in air-dried, Wright-stained blood-smears described by Yeager (1945) from heat-fixed Prodenia can be recognized in unfixed and unstained preparations with the phase microscope. With only one exception, the bloodcells do not undergo extensive transformations in vitro for at least one hour. Hence, the blood-cells may be accurately identified and differentially counted by phase microscopy. Two kinds of blood-cells undergo marked alterations in appearance on heatfixation, whereas the other types remain mostly unmodified. One type of blood-cell, the oenocytoid, typically undergoes a marked change in character in vitro in unfixed blood. Several changes in Yeager's classification of insect blood-cells are proposed, and it is suggested that this new system will be found sufficient for identifying and comparing blood-cells in the different orders of insects.






© The Company of Biologists Ltd 1959