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Quarterly Journal of Microscopical Science, Vol s3-100, 279-284, Copyright © 1959 by Company of Biologists

The Nature of a Chromosomal Phospholipid

J. CHAYEN 1, P. B. GAHAN 1, and L. F. LA COUR 1

1 Imperial Cancer Research Fund, Department of Pathology, Royal College of Surgeons, Lincoln's Inn Fields, London, W.C. 2, and John Innes Horticultural Institution, Bayfordbury, Hertford, Herts

It has been found that the heterochromatic region of interphase nuclei of plant roots and calf thymus, fixed in formaldehyde solution, do not stain for phospholipid as they do after Lewitsky's fixative. It has been shown that this is probably due to the extraction of a protein-phospholipid complex. Some lipid-like material has been purified from the used formalin and shown to contain glycerophosphate. The nature of the association between the lipid and protein is discussed.






© The Company of Biologists Ltd 1959