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Quarterly Journal of Microscopical Science, Vol s3-101, 251-254, Copyright © 1960 by Company of Biologists

The Initial Cooling of Tissues in the Freezing-Drying Technique

I. ZLOTNIK PH.D., B.V.Sc., M.R.C.V.S.1

1 Moredun Research Institute, Gilmerton, Edinburgh

When a mixture of dry ice and pentane is subjected to high vacuum for one hour the temperature of the mixture is reduced to -125° C.

The low temperature of the dry ice/pentane mixture persists for about 1/2 h and rises only very slowly thereafter.

The chilled dry ice mixture when used for the initial cooling of tissues produces good cytological preservation.






© The Company of Biologists Ltd 1960