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Quarterly Journal of Microscopical Science, Vol s3-103, 17-23, Copyright © 1962 by Company of Biologists

The Fine Structure of the Normal Chorio-Allantoic Membrane of the Chick-Embryo

S.R. S. RANGAN 1 and SATYAVATI M. SIRSAT 1

1 Indian Cancer Research Centre, Bombay 12, India

The chorio-allantoic membranes of White Leghorn chick embryos at 10 to 12 days after laying were fixed in Palade's buffered osmium tetroxide or in Luft's potassium permanganate.

After fixation in these two ways the general appearance is similar, but there are differences in certain tissue elements. Cell membranes are well preserved after fixation in KMnCv Certain lipid inclusions in the cytoplasm of the cells of the allantoic layer are well seen after fixation by OsO4, but not after KMnO4; in their places empty vacuoles are seen.

Details of the structure of the red blood-corpuscles are more clearly seen after fixation in KMnO4.






© The Company of Biologists Ltd 1962