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Quarterly Journal of Microscopical Science, Vol s3-103, 205-209, Copyright © 1962 by Company of Biologists

The Demonstration of Lysosomes by the Controlled Temperature Freezing-Sectioning Method

LUCILLE BITENSKY 1

1 Department of Pathology, Royal College of Surgeons of England, Lincoln's Inn Fields, London, W.C. 2

Lysosomes, as isolated and defined by biochemists, are particles in which acid lytic enzymes are contained by a lipoprotein membrane; they are inert until the membrane has been rendered permeable. Previously it has not been possible to demonstrate cytochemically organelles which meet all these criteria, since all the preparatory procedures have affected the membrane permeability. The controlled temperature freezing-sectioning method has permitted the demonstration of granules which are inactive for acid phosphatase until subjected to agents that will disrupt lipid-protein structures, such as heat, formalin, and ‘triton X-100’. Hence such organelles may be identified with the biochemists lysosomes.






© The Company of Biologists Ltd 1962