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Quarterly Journal of Microscopical Science, Vol s3-103, 287-296, Copyright © 1962 by Company of Biologists

Experiments on Fixation for Electron Microscopy 2. Alkalized Osmium Tetroxide

S. K. MALHOTRA 1

1 Cytological Laboratory, Department of Zoology, University Museum, Oxford

The effect of fixation with osmium tetroxide solution, made alkaline by the addition of potassium acetate, was studied by electron microscopy. Exocrine cells of the pancreas and the cells of the first (‘proximal’) convoluted tubules of the kidney of the mouse were used as test-objects. Partially prepolymerized methacrylate was used for embedding. The preservation of the various cell inclusions was similar to what is generally produced after fixation with Palade's buffered osmium tetroxide.






© The Company of Biologists Ltd 1962