spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by WILLIAMS, T.
Right arrow Search for Related Content
PubMed
Right arrow Articles by WILLIAMS, T.

Quarterly Journal of Microscopical Science, Vol s3-103, 297-310, Copyright © 1962 by Company of Biologists

The Staining of Nervous Elements by the Bodian Method

II. The Effects of Some Variable Factors at the Impregnation Stage

TERRY WILLIAMS 1

1 Department of Anatomy, The University, Manchester 13

In paraffin sections of tissues containing much smooth muscle, nerve-fibres are difficult to demonstrate by silver techniques. In pilot trials Bodian's activated protargol technique proved most promising for stomach sections. From a fixed Bodian-type schedule, the effects of variation in pH, time, and temperature of impregnation were studied separately and together. These experiments provided means of determining optimal levels for some of the variables in the process. The impregnation time required for optimal staining varies according to the temperature and initial pH. Stirring, or agitation of the slides, at intervals of a few hours has been found to be a valuable aid to even staining, and if this has been done it is possible to use the stage of clearing of the fluid as an end-point indicator.






© The Company of Biologists Ltd 1962