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Quarterly Journal of Microscopical Science, Vol s3-104, 101-106, Copyright © 1963 by Company of Biologists

The Fine Structure Produced in Cells by Primary Fixatives

1. Mercuric Chloride

JOHN R. BAKER 1 and BARBARA M. LUKE 1

1 Cytological Laboratory, Department of Zoology, University Museum, Oxford

The exocrine cells of the mouse pancreas were fixed in mercuric chloride solution, embedded in plexigum, and examined by electron microscopy. The cytoplasm was found to be coagulated as a continuous substance containing innumerable subspherical cavities, mostly between 40 and 200 mµ in diameter and separate from one another. The zymogen granules were preserved, but no trace remained of mitochondria or Golgi apparatus. The nuclear sap was coagulated as a coarse network with thickenings at the nodes. Lumps of electron-dense material (? DNA) were present at the periphery of the nucleus and round the nucleolus.

The proteins of the cell appear to have been fixed by mercuric chloride, but the membranous constituents, which rely for their form on a phospholipid component, are not clearly recognizable. The lipids have presumably been lost during dehydration and embedding.






© The Company of Biologists Ltd 1963