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Quarterly Journal of Microscopical Science, Vol s3-104, 123-127, Copyright © 1963 by Company of Biologists

Experiments on Fixation for Electron Microscopy

3. A Note on Fixation in Acidified Osmium Tetroxide

S. K. MALHOTRA 1

1 Cytological Laboratory, Department of Zoology, University Museum, Oxford

The effect of fixation with acidified solutions of osmium tetroxide (pH 1.5 to 3.5 has been studied on the first (proximal) convoluted tubule cells of the kidney and the pancreatic exocrine cells of the mouse, by electron microscopy. Partially prepolymerized methacrylate was used for embedding. The various membranous structures and the ribosomes retain their individuality even after prolonged fixation in solutions containing 5% acetic acid (pH 1.5). However, the mitochondrial matrix and the ground cytoplasm are not preserved; the zymogen granules are also partially washed out.






© The Company of Biologists Ltd 1963