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Quarterly Journal of Microscopical Science, Vol s3-105, 363-366, Copyright © 1964 by Company of Biologists

A Silver-Schiff Reaction for Cytological Studies: Light Microscope Observations

JOHN H. D. BRYAN 1

1 Department of Zoology and Entomology, Iowa State University, Ames, Iowa, U.S.A.

A method is presented in which a complex metal-ion (ammoniacal silver) is substituted for leuco-basic fuchsin in the well-known Schiff reaction for aldehydes. The reagent is reduced to metallic silver by tissue aldehydes and submicroscopic particles of the metal are deposited at the reactive sites. When the reagent is used in the Feulgen reaction chromosomes may be ‘stained’ in a specific manner to show the localization of deoxyribonucleic acid. For such a purpose, fixation should be carried out with acetic acid and alcohol mixtures rather than with solutions containing formalin or dichromate; these latter substances preserve more of the tissue lipids and thus complicate the issue by producing cytoplasmic staining (plasmal reaction).






© The Company of Biologists Ltd 1964