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Quarterly Journal of Microscopical Science, Vol s3-105, 379-380, Copyright © 1964 by Company of Biologists

Serial Sectioning of Refractory Locust Eggs

D. S. ANDERSON 1

1 Department of Zoology, Birkbeck College, University of London, Malet St., London, W.C. 1

A technique is described for sectioning the yolky tissue of locust eggs. It is a modified version of a method evolved by Griffiths and Carter and successfully used on reptile eggs. The fixative is dioxan saturated with picric acid, with 5% formic acid and 10% formaldehyde. It rapidly penetrates the yolk and minimizes hardening. Dehydration in cellosolve is followed by clearing in benzene. After embedding in wax the block is immersed in 5% tergitol ethane-diol solution, and 12 h later good serial sections are obtained.






© The Company of Biologists Ltd 1964