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Quarterly Journal of Microscopical Science, Vol s3-106, 193-196, Copyright © 1965 by Company of Biologists

The Histochemical Demonstration of Alkaline Phosphatase in Unfixed Frozen Sections

LUCILLE BITENSKY 1 and S. COHEN 2

1 Department of Pathology, Royal College of Surgeons of England, Lincoln's Inn Fields, London, W.C. 2 and Shotley Bridge General Hospital County Durham
2 Department of Pathology, Royal College of Surgeons of England, Lincoln's Inn Fields, London, W.C. 2 and Shotley Bridge General Hospital, County Durham

Although, ideally, enzyme histochemistry should be performed on unfixed sections, so as to avoid potential artifacts inherent in chemical fixation, it is usually considered lecessary to fix sections so as to stop the ‘soluble’ enzymes from becoming lost into the incubation medium. However, provided that suitable frozen sections are used, alsaline phospatase can be demostrated fully in unfixed sections, even though this is typically a ‘soluble’ enzyme.






© The Company of Biologists Ltd 1965