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Quarterly Journal of Microscopical Science, Vol s3-89, 351-358, Copyright © 1948 by Company of Biologists

A Simple Method for Phase-Contrast Microscopy

D. A. KEMPSON 1, O. L. THOMAS 1, and JOHN R. BAKER 1

1 Department of Zoology and Comparative Anatomy, Oxford

A method of phase-contrast microscopy is described, not involving the use of special objectives or condensers. A method for making the phase-plate carrying a raised annulus is described. A large annular source of light is focused by the condenser of the microscope in a plane slightly below the object. The phase-plate is placed in the conjugate focus of this plane, just above the back lens of the objective.

Note:

Beit Memorial Medical Research Fellow.






© The Company of Biologists Ltd 1948