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Quarterly Journal of Microscopical Science, Vol s3-90, 57-66, Copyright © 1949 by Company of Biologists

A Dye Phosphate for the Histo- and Cytochemical Demonstration of Alkaline Phosphatase, with some Observations on the Differential Behaviour of Nuclear and Extranuclear Enzymes

A. LOVELESS 1 and J. F. DANIELLI 1

1 Chester Beatty Research Institute, Royal Cancer Hospital, London, S.W. 3

1. A new method is described for the cyto- and histochemical demonstration of alkaline phosphatase (monoesterase), a synthetic substrate p-nitrobenzene-azo-4{alpha}-naphthol-phosphate being employed as the sodium salt. Two methods for preparing a solution of this substance are described, and optimal conditions for enzymic hydrolysis of the ester worked out by the use of kidney sections.

2. Experiments are also described in which the substrate has been used to demonstrate phosphatase activity in calcifying tissues of dogfish and rat.

3. The results of the experiments with rat kidney indicate that traces of end-products are necessary before the enzyme can attack this substrate.

4. When results obtained with the new substrate are compared with those obtained with {beta}-glycerophosphate, it is found that some sites display more activity towards one substrate than to the other.

5. The cytochemical localization of phosphatase with the new substrate is not as precise as with {beta}-glycerophosphate.






© The Company of Biologists Ltd 1949