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Quarterly Journal of Microscopical Science, Vol s3-92, 393-402, Copyright © 1951 by Company of Biologists

The Histochemical Demonstration of Keratin by Methods involving Selective Oxidation

A. G. EVERSON PEARSE 1

1 Department of Pathology, Postgraduate Medical School, Ducane Road, London, W. I2

1. Oxidation of tissues with performic acid gives rise to histochemically detectable reaction products particularly in two classes of material. These are keratin and lipoids of the phosphatide class.

2. Three methods have been evolved for visualizing the effect of performic acid on cystine-containing structures; two of these (performic acid/Schiff and perfoiTnic acid/cobalt nitrate) also record the effect on lipoids.

3. An attempt has been made to elucidate the chemistry of the reactions and it is suggested that oxidation of cystine in the tissues gives rise not only to cysteic acid (alanine-beta-sulphonic) but to another acid (alanine-beta-sulphinic). The latter is responsible for the positive reaction with Schiff's solution.

4. The Schiff reaction with performic acid oxidized lipoids is due to the formation of substances giving the reactions of aldehydes. It is possible that similar groups may be produced from lipoid molecules by periodic acid oxidation and that these and not polysaccharides (1.2 glycols) are responsible for the periodic acid-Schiff reaction in such cases.






© The Company of Biologists Ltd 1951