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Quarterly Journal of Microscopical Science, Vol s3-95, 323-325, Copyright © 1954 by Company of Biologists

Lead Tetra-Acetate/Schiff Tests in Histochemistry

W. G. BRUCE CASSELMAN 1

1 Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada, and the Cytological Laboratory, Department of Zoology and Comparative Anatomy, University Museum, Oxford

Certain polysaccharides, such as starch and glycogen, do not give consistently positive or negative reactions with all lead tetra-acetate/Schiff techniques. This depends upon the conditions under which the oxidant is used. A simple glacial acetic acid solution of lead tetra-acetateis least active but most specific. Added potassium acetate acts as a catalyst. Dilution with water not only increases the activity of the reagent but also decreases the specificity of the test.






© The Company of Biologists Ltd 1954