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Quarterly Journal of Microscopical Science, Vol s3-96, 295-299, Copyright © 1955 by Company of Biologists

A Study of the Kultschitzky (Argentaffin) Cell with the Electron-microscope, after Fixation by Osmium Tetroxide

A. C. CHRISTIE 1

1 Royal Cancer Hospital, London. Present address, Royal Hospital for Women, Paddington, Sydney, Australia

1. The Kultschitzky cells of the alimentary canal of man and the guinea-pig were studied with the light-microscope, buffered osmium tetroxide being used as fixative.

2. The Kultschitzky cells of the guinea-pig were shown by the electron-microscope to contain spheroidal granules having a maximum diameter of 0.3µ. These granules are considerably darkened by fixation for only 4 hours in 1 % osmium tetroxide solution.

3. The opinion that the granules of the Kultschitzky cell are only an artifact of formaldehyde fixation is denied.






© The Company of Biologists Ltd 1955