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Quarterly Journal of Microscopical Science, Vol s3-98, 151-154, Copyright © 1957 by Company of Biologists

The Interference Microscope as a Refractometer for Liquids

S. IVERSEN 1 and F. H. SMITH 1

1 Department of Anatomy, Royal College of Surgeons of England, London, W.C. 2, and Research Laboratories of Messrs. Charles Baker, Croydon

Determination of the refractive index of liquids by the interference microscope was made possible by the use of a specially constructed holder for the liquid. This ensures a permanent lateral separation between the reference and the object areas and, as the depth of the object area is continuously increasing, a system of fringes is observed. The spacing of the fringes is a function of the refractive index of the liquid. By determining this spacing, a measurement of the refractive index is obtained. An accuracy of at least 0.001 in refractive index is obtainable by a single measurement






© The Company of Biologists Ltd 1957