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Quarterly Journal of Microscopical Science, Vol s3-98, 493-498, Copyright © 1957 by Company of Biologists
1 Department of Zoology, The Queen's University of Belfast
The stages of spermateleosis and the structure of the spermatozoon of Fasciola hepatica were studied in smears of fixed material and in preparations of living tissue examined under phase-contrast microscopy.
Smears were fixed in Bouin's picro-formol and in Flemming without acetic. Smears were also prepared according to the Feulgen technique.
The nucleus of the late spermatid undergoes elongation and grows out from the distal end of the cell. An extension of cytoplasm gives rise to the sperm-tail or flagellum. The spermatozoon becomes free of the greater part of the cytoplasm of the spermatid; the latter remains as a residual mass.
The spermatozoon consists of a nucleus and a flagellum. The latter is probably composed of an axial filament surrounded by a thin sheath of cytoplasm.