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Quarterly Journal of Microscopical Science, Vol s3-99, 453-457, Copyright © 1958 by Company of Biologists

Unmasking of Sudanophil Lipid in the Testis of the House-cricket, Acheta domesticus

BLANCHE-P CLAYTON 1

1 Cytological Laboratory, Department of Zoology, University Museum, Oxford

1. When the testis of Acheta domesticus is fixed in Flemming's fluid and embedded in gelatine, Sudan black reveals scarcely any lipid in the externum of the acroblast.

2. It can be shown that the externum of the acroblast contains much lipid in a masked (bound) form.

3. Experiments were performed to find what substances were effective, after Flemming fixation, in unmasking the lipid of the externum and thus making it colourable by Sudan black.

4. The following substances were found to act as unmasking agents: ethanol, dioxane, phenol, resorcinol, hydroquinone, pyrogallol, tannic acid.

5. Under the conditions of the experiment, the most effective unmasking agents were 90% ethanol and 5% hydroquinone.






© The Company of Biologists Ltd 1958