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Addition and correction: the NF-{kappa}B-like DNA binding activity observed in Dictyostelium nuclear extracts is due to the GBF transcription factor

François Traincard1, Eleonora Ponte1, Jason Pun2, Barrie Coukell2 and Michel Veron1

1 Unité de Régulation enzymatique des Activités cellulaires, CNRS FRE 2364, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
2 York University, 4700 Keele Street, Toronto, Ontario, M3J 1P3, Canada.



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  		Fig. 1. Sequences of oligonucleotides used in gel retardation experiments. Sequences of the GCR, Car3 and Ig{kappa} oligonucleotides as well as consensus sequences of NF-{kappa}B (NF-{kappa}B cons.) and GBF (GBF cons.) DNA binding sites are shown. The GBF binding sites are underlined. N is any nucleotide; Y is any pyrimidine; P is any purine.

 


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  		Fig. 2. Gel retardation of GCR and Car3 oligonucleotides by p50 and Dictyostelium cell extracts. Gel shift assays performed with [32P] labelled GCR, Car3 and Ig{kappa} oligonucleotides in the presence of 100 ng of recombinant mouse p50 (row 1), 16-hour-developed Dictyostelium nuclear extracts (row 2) and cytoplasmic extracts (row 3). The experiments were performed in the absence (Fig. 2A) or in the presence (Fig. 2B) of a 25-fold excess of non-labelled GCR, Car3 or Ig{kappa} oligonucleotides used as competitors (italics). Gel shifts were performed as described previously (Traincard et al., 1999), except for experiments performed with Ig{kappa} as labelled probe in which threefold the amount of Dictyostelium extract was used. n.d., not done.

 

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© The Company of Biologists Ltd 2001