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Cytoskeletal control of fibroblast length: experiments with linear strips of substrate

Elina M. Levina, Margarita A. Kharitonova, Yuri A. Rovensky and Jury M. Vasiliev

Cancer Research Center of Russian Federation, 115478 Moscow, Russia



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  		Fig. 1. Two alternative models of cell length control. Limitation of transverse cell spreading is compensated (gray arrow) or not compensated (black arrow) for by an increase in longitudinal spreading.

 


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  		Fig. 2. Dynamics of average cell length changes in M19 fibroblasts on the usual planar substrates and on the narrow strips.

 


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  		Fig. 3. Mouse embryo fibroblasts on the control substrate (a) or on the substrate with narrow adhesive strips (b). DIC microscopy. Bars, 20 µm.

 


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  		Fig. 4. AGO 1523 cells on the control substrate (a) or on the substrate with narrow adhesive strips (b, c and d). Control cells (a, b), short colcemid-treated cells (c) and long cytochalain-treated cells (d). Phase contrast microscopy. Bar, 30 µm.

 


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  		Fig. 5. AGO 1523 cells on the substrate with narrow adhesive strips. Fluorescent microscopy after staining for actin (a) and paxillin (b). Bar, 30 µm.

 


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  		Fig. 6. FBT cells on the control substrate (a) or on the substrate with narrow adhesive strips (b). DIC microscopy. Bar 15 µm.

 

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