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Failure of normal adult Leydig cell development in androgen-receptor-deficient mice

Peter J. O'Shaughnessy*, Heather Johnston, Louise Willerton and Paul J. Baker

Institute of Comparative Medicine, University of Glasgow Veterinary School, Bearsden Rd, Glasgow G61 1QH, UK



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  		Fig. 1. Light micrographs showing morphology of testes from normal, AR-null and cryptochid adult mice. Bar, 50 µm.

 


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  		Fig. 2. Leydig cell number during development in normal mice and in AR-null animals. Cryptorchid controls are included for the adult group. Within each age group different letter superscripts indicate a significant difference in cell number (P<0.05). Results show means±s.e.m. of between three and five animals in each group.

 


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  		Fig. 3. Expression of specific mRNA levels in testes of normal mice and AR-null mice. The level of each mRNA species is expressed relative to an external standard (luciferase). Within each age group, different letter superscripts indicate a significant difference in mRNA levels (P<0.05). Results show means±s.e.m. of between four and six animals in each group. (A) Genes expressed at normal or increased levels in AR-null mice. 3ßHSD I, 3ß-hydroxysteroid dehydrogenase type I; P450scc, cytochrome P450 side chain cleavage; LH-R, luteinising hormone receptor; StAR, steroidogenic acute regulatory protein, Ren 1, renin type 1; TSP-2, thrombospondin type 2. (B) Genes with barely detectable levels of expression in AR-null mice. 17ßHSD III, 17ß-hydroxysteroid dehydrogenase type III; PGD, prostaglandin D-synthetase; 3ßHSD VI, 3ß-hydroxysteroid dehydrogenase type VI. (C) Genes with reduced levels of expression in AR-null mice. RLF, relaxin-like factor; GST5-5, glutathione S-transferase 5-5; P450c17; cytochrome P450 17{alpha}-hydroxylase; EH, epoxide hydrolase; EST, estrogen sulfotransferase.

 


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  		Fig. 4. Activity of 5{alpha}-reductase in testes from normal or AR-null mice of different ages. Enzyme activity is expressed per testis as pmol/minute. Within each age group different letter superscripts indicate a significant difference in enzyme activity (P<0.05). Results show means±s.e.m. of between three and six animals in each group.

 

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© The Company of Biologists Ltd 2002