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Fig. 6. Polarized distribution of the tight junction associated protein, ZO-1, in
aggregates of Pax3flag-expressing Saos-2 cells. The formation of
apico-basal epithelial type cell polarity was assessed by examining the
distribution of ZO-1 by indirect immunofluorescence confocal microscopy. The
distribution of ZO-1 in control infected (a-d,m) or
Ad-Pax3flag-infected (e-l,n) cells was examined at three days
postinfection. Optical sections through the regions indicated are depicted in
a-c, e-h and i-l. Panels e-h are representative of the ZO-1 distribution in
single layered aggregates, and (i-l) its distribution in multi-layered (this
series depicts a two-layered aggregate) aggregates of
Ad-Pax3flag-infected cells. (j) Optical section through the
interface between the apex of the layer of cells attached to the substratum
and the basal regions of the second layer of cells. (m,n) XZ-projections of
the series presented in a-d and e-f, respectively. Note that in
Ad-Pax3flag-infected cells, ZO-1 junctional staining is restricted
to the apex of lateral sites of cell-cell contact (arrow in n), and in
multi-layered aggregates the junctional staining is strongest at the apex of
exposed cells (compare j and k). Bars, 20 µm (a,e,i). Bars, 10 µm
(m,n).
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-catenin (h,i) and cadherin-11 (a-c) were analyzed by indirect
immunofluorescence confocal microscopy at three days postinfection. Bars, 20
µm.
