QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

doi: 10.1242/10.1242/jcs.00196

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhimulev, I. F. Articles by Volkova, E. I. Search for Related Content PubMed PubMed Citation Articles by Zhimulev, I. F. Articles by Volkova, E. I. Social Bookmarking                         What's this?

Overexpression of the SuUR gene induces reversible modifications at pericentric, telomeric and intercalary heterochromatin of Drosophila melanogaster polytene chromosomes

Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Novosibirsk, 630090, Russia

View larger version (83K): [in a new window]   Fig. 1. Cell specificity of expression and overexpression of the Sgs3 promoter in the salivary gland cells. Staining in the distal (D) parts and absence of staining in the proximal (P) parts of the salivary gland in Sgs3-Gal4 UAS-lacZ larvae (A-C). No expression was seen in young (about 100 hours) larvae (A). The beginning of expression was seen in the salivary glands of older (B) and late (C) larvae. Binding of antibodies against SuUR protein in polytene chromosomes of distal part of salivary gland of young SuUR/SuUR Sgs3-Gal4 UAS-SuUR+ larvae: immunofluorescence (D) and phase contrast (E). Arrows point to a smaller polytene chromosome in the proximal part of the salivary gland that does not bind the antibodies. Binding of antibodies against SuUR protein in polytene chromosomes of the distal part of salivary gland of wildtype (F,G) shown by immunofluorescence (F) and phase contrast (G). Recognition of an additional band in the SuUR/SuUR Sgs3-Gal4 UAS-SuUR+ larvae by the SuUR antibodies on western blots (H)

View larger version (105K): [in a new window]   Fig. 2. Proximal part of the polytene 3R chromosome of SuUR+/SuUR+ Sgs3-GAL4 UAS-SuUR+ young larva (A) and late prepupa (B): small capsules and swellings in regions 83DE, 84AB, 84D, 86C, 86D, 87B, 87D, 87F and 88E are marked.

View larger version (90K): [in a new window]   Fig. 3. Swellings in the distal part of the chromosome 2R of Oregon R control stock (A) and SuUR+/SuUR+ Sgs3-GAL4 UAS-SuUR+ young larvae (B), late larva (C) and late prepupa (D). The chromosome shown in E was taken from prepupa that developed till the 0 hours prepupal stage at 29°C followed by a 15 hour development at 18°C. Asterisks in A mark regions of late replication.

View larger version (125K): [in a new window]   Fig. 4. Decondensation and swelling of pericentric heterochromatin regions in SuUR+/SuUR+ Sgs3-GAL4 UAS-SuUR+ (B) in comparison with the chromocenter of the Oregon-R control line (A). Arrows show chromocentral regions.

View larger version (21K): [in a new window]   Fig. 5. Swelling and capsule formation in regions demonstrating different characteristics of heterochromatin. Abscissa: regions of late replication (1), SuUR (2) and PcG (3) protein localization, weak points in wildtype (2xSuUR+) (4) and in line with extra copies of SuUR [(4-8) x SuUR+] (5). Ordinate: number of regions demonstrating different characteristics (total height of columns) of intercalary heterochromatin and number of swellings or capsule formation regions among them (blue part of bars).

View larger version (106K): [in a new window]   Fig. 6. Failure to incorporate [3H]uridine into swellings in 83DE, 84AB and 84D regions as well as in some unidentified regions (indicated by arrows in A) in chromosomes of SuUR/SuUR Sgs3-GAL4 UAS-SuUR+ prepupae. (C) Incorporation of the [3H]uridine into regular puffs 62C, 62E and 63E in wild-type polytene chromosomes.

View larger version (85K): [in a new window]   Fig. 7. Structure of swellings under an electron (A,B) and a fluorescent (C,D) microscope. Part of the swelling in 56F (rectangular in A) is shown at higher magnification in B. An external envelope of the bubble is indicated by arrows with crosses; inner envelopes around small bubbles are indicated by straight arrows; and open thick arrow indicate the matrix within the bubble. A fluorescent Hoechst-33258-stained regular chromosome puff 71CF of the Oregon R strain (C) and swellings in regions designated by arrows in chromosomes of SuUR/SuUR Sgs3-GAL4 UAS-SuUR+ prepupae (D) are shown. Scales represent 1 µm (A) and 0.1 µm (B).

View larger version (75K): [in a new window]   Fig. 8. Localization of SuUR antibodies in polytene chromosomes of the SuUR+/SuUR+ Sgs3-GAL4 UAS-SuUR+ prepupae after overexpression of the SuUR+ gene. The swollen chromocenter (arrow), small capsules (21D, 22A, 22B and 24D) and big swellings (75C, 74A, 71C, 67D and others) are free of the antibodies.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

Twitter