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First published online 3 June 2003
doi: 10.1242/jcs.00601

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The Arabidopsis SYN1 cohesin protein is required for sister chromatid arm cohesion and homologous chromosome pairing

¹ The Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA
² Department of Botany, Miami University, Oxford, OH 45056, USA

View larger version (65K): [in a new window]   Fig. 1. Fluorescence in situ hybridization of BAC probes to chromosome arms in wild-type (A-D) and syn1 (E-H) meiocytes. Hybridization of BAC probes was detected with fluorescein-labeled streptavidin (green). Chromosomes were stained with PI (red). (A,E) Early leptotene; (B,F) pachytene; (C,G) early diplotene; (D,H) prometaphase. Normal-appearing chromosomes at zygotene, pachytene, diplotene or diakinesis were never observed in syn1 plants. Therefore, assignment of the stage of the cell cycle is approximate and based on chromosome morphology and the stage of surrounding cells in the anther. Bar, 5 µm.

View larger version (86K): [in a new window]   Fig. 2. Fluorescence in situ hybridization of centromere probes to chromosomes in syn1 meiocytes. Hybridization of a centromere probe (pAL1) was detected with fluorescein-labeled streptavidin (green). Chromosomes were stained with PI (red). (A) An example of an early leptotene meiocyte showing six centromere signals; (B) zygotene; (C) diplotene; (D) diakinesis; (E) metaphase; (F) telophase I. Assignment of the stage of the cell cycle is approximate and based both on chromosome morphology and the stage of surrounding cells in the anther. Bar, 5 µm.

View larger version (83K): [in a new window]   Fig. 3. Fluorescence immunolocalization of SYN1 antibody (green) on meiotic spreads of wild type Arabidopsis chromosomes counterstained with PI (red). (A) Interphase; (B) early leptotene; (C) leptotene; (D) early zygotene; (E) zygotene; (F) pachytene; (G) late pachytene; (H) early diplotene; (I) diplotene; (J) diakinesis; (K) prometaphase; (L) metaphase; (M) anaphase I; (N) telophase I; (O) meiotic interphase II. Bar, 5 µm.

View larger version (53K): [in a new window]   Fig. 4. Fluorescence immunolocalization of SYN1 antibody (B,D,F,H,K; green) on meiotic spreads of wild type Arabidopsis chromosomes (A,C,E,G,J) counterstained with PI (red). (A,B) Diplotene; (C,D) diakinesis; (E,F) metaphase I; (G,H) anaphase I; (J,K) meiotic interphase II. Bar, 5 µm.

View larger version (86K): [in a new window]   Fig. 5. Simultaneous immunolocalization of SYN1 antibody (green) and fluorescence in situ hybridization of centromere probes (red) to meiotic chromosomes. (A) Zygotene; (B) early pachytene; (C) late pachytene; (D) early diplotene; (E) diakinesis; (F) prometaphase; (G) metaphase I; (H) early anaphase I. Bar, 5 µm.

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