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First published online 2 September 2003
doi: 10.1242/jcs.00715

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Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation

Kobe Vlieghe1, Marnik Vuylsteke1, Kobe Florquin1, Stephane Rombauts1, Sara Maes1, Sandra Ormenese2, Paul Van Hummelen3, Yves Van de Peer1, Dirk Inzé1,* and Lieven De Veylder1

1 Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, B-9052 Gent, Belgium
2 Laboratory of Plant Physiology, University of Liege, B-4000 Liège, Belgium
3 VIB MicroArray Facility, Gasthuisberg, Onderzoek en Navorsing, B-3000 Leuven, Belgium



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  		Fig. 1. Volcano plot of significance against effect. Each x represents one of the 4,571 genes, with the negative log10 of the P value from the gene model plotted against the difference between least-square means for the genotype effect. The horizontal line represents the test-wise threshold of P=0.05. The two vertical reference lines indicate a twofold cut-off for either repression or induction.

 


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  		Fig. 2. Verification of microarray analysis by RT-PCR. RT-PCR analysis was carried out under linear amplification conditions. The actin 2 gene (ACT2) was used as loading control. GS, glutamine synthetase; GOGAT, glutamate synthase; NiR, nitrite reductase.

 


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  		Fig. 3. Sources of {alpha}-ketoglutarate in the E2Fa-DPa-overproducing cells. Genes encoding enzymes shown in red and green are up-regulated or down-regulated in the E2Fa-DPa versus wild-type plants, respectively. Products indicated in blue act as precursors for nucleotide biosynthesis. {alpha}-KG, {alpha}-ketoglutarate; GOGAT, glutamate synthase; GS, glutamine synthetase; NIA2, nitrate reductase; NiR, nitrite reductase.

 

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© The Company of Biologists Ltd 2003