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First published online 30 November 2004
doi: 10.1242/jcs.01580

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Role of myosin VIIa and Rab27a in the motility and localization of RPE melanosomes

Daniel Gibbs¹, Sassan M. Azarian¹, Concepcion Lillo¹, Junko Kitamoto¹, Adriana E. Klomp¹, Karen P. Steel^2,*, Richard T. Libby^2, and David S. Williams^1,

¹ Departments of Pharmacology and Neurosciences, UCSD School of Medicine, La Jolla, CA 92093-0912, USA
² MRC Institute of Hearing Research, University Park, Nottingham, NG7 2RD, UK

View larger version (120K): [in a new window]   Fig. 1. Light micrographs of purified sheets of RPE cells from (A) shaker1 control (Myo7a+/4626SB) and (B) shaker1 mutant (Myo7a4626SB/4626SB) mouse retinas. Apical processes are evident as lightly stained region below the cell bodies (indicated by square brackets). In the control RPE, melanosomes are present in the apical processes, whereas the apical processes of the mutant RPE are devoid of melanosomes. Bar, 10 µm.

View larger version (73K): [in a new window]   Fig. 2. RT-PCR of purified mouse RPE cells. (A) Representative RT-PCR of mRNA expression of Rab27a, Rab27b, Melanophilin (Mlph), Myrip, Myo7a and Myo5a in purified mouse RPE cells, retina and cerebellum. Control, PCR reaction without added DNA. The position of DNA size-standards in bp is given at the left. (B) Representative RT-PCR of mRNA expression of different exophilins in purified mouse RPE cells, retina and cerebellum. 1, Exophilin 1-Rabphilin 3a; 2, Exophilin2-Granuphilin-Sytl4; 3, Exophilin3-Melanophilin-Slac2-a; 4, Exophilin4-Sytl2; 5, Exophilin5-Slac2-b, 6, Exophilin6-Sytl3; 7, Exophilin7-Sytl1; 8, Exophilin8-Myrip-Slac2-c; 9, Exophilin9-Sylt5. Control, PCR reaction without added DNA. The position of DNA size-standards in bp is given at the left.

View larger version (157K): [in a new window]   Fig. 3. Light micrographs of semithin sections of the RPE and outer segments from mice that were (A) shaker1 control (Myo7a+/4626SB), (B) shaker1 mutant (Myo7a4626SB/4626SB), (C) dilute lethal (Myo5ad-l/d-l), (D) Ames' waltzer (Pcdh15av-3J/av-3J), (E) Snell's waltzer (Myo6sv/sv), (F) waltzer (Cdh23v-2J/v-2J), (G) dilute lethal (Myo5ad-l/d-l) plus shaker1 heterozygote (Myo7a+/4626SB), (H) Snell's waltzer (Myo6sv/sv) plus shaker1 heterozygote (Myo7a+/4626SB) and (I) waltzer (Cdh23v-2J/v-2J) plus shaker1 heterozygote (Myo7a+/4626SB). Genotypes are indicated on each panel. All images are the same magnification (bar, 10 µm).

View larger version (92K): [in a new window]   Fig. 4. Light (A,B) and electron (C,D) micrographs of the RPE from ashen mice. (A,C) Control (Rab27a+/ash), (B,D) mutant (Rab27aash/ash). The mice were on the C3H/HeSn background, which includes the rd1 mutation. Retinas were obtained from 14-day-old animals. No photoreceptor outer segments are evident owing to the initiation of photoreceptor degeneration, however, at this stage, the RPE remains unaffected by the degeneration. Arrows in A and B indicate the apical region of the RPE, which is completely devoid of melanosomes in the mutant. Arrows in C indicate melanosomes partially contained within the apical processes. Arrow in D indicates the apical processes, with melanosomes some distance away. A is the same magnification as B (bar, 10 µm) and C is the same as D (bar, 100 nm).

View larger version (62K): [in a new window]   Fig. 5. Mouse retinal cryosections immunolabeled with antibodies against (A) myosin VIIa or (B) myosin Va (both red), and also labeled with BODIPY-FL-phallacidin (green) to indicate actin filaments. A region of overlap is evident as a yellow band. Myosin Va is also evident in the photoreceptor inner segments. R, RPE; O, photoreceptor outer segments; I, photoreceptor inner segments. Bars, 10 µm. (C) RPE subcellular fractionation and immunoblot analysis. Left: Schematic of the fractionation procedure for purified pig RPE cells. Right: Analysis of RPE fractions. Each fraction was obtained from the same amount of starting material. Top strip: unstained stacking gel showing melanin content of each fraction, as an indication of the distribution of melanosomes. Lower strips: immunoblot labeled with myosin VIIa, myosin Va, and Hsp60 antibodies. All strips are from the same gel. Melanosomes are enriched in P2 and then in GP (and to a lesser extent in GI), following gradient separation. (GP, fraction from bottom of gradient; GI, fraction from interface; R, remainder.) Nuclei, as indicated by Trypan Blue staining were absent from GP. Labeling with Hsp60 antibody (a mitochondrial marker) indicates that mitochondria were depleted from P2 and GP.

View larger version (164K): [in a new window]   Fig. 6. Electron micrograph of the RPE from a shaker1 control (Myo7a+/4626SB) mouse retina immunolabeled with myosin VIIa antibody (10 nm gold particles). The apical region is lower right and the basal region is upper left. In the apical region of the RPE cell, melanosomes are oriented along the axis of the apical processes. Myosin VIIa is present both on the melanosome membrane (circles) and unassociated with the melanosomes, elsewhere in the cytoplasm (arrows). Insert is a magnification of the square in the picture, showing two gold particles associated with the melanosome membrane. Bar, 300 nm.

View larger version (14K): [in a new window]   Fig. 7. (A) Graph showing the percentages of gold particles, representing myosin VIIa labeling, distributed on the melanosome membrane versus those unassociated with the melanosomes but present elsewhere in the RPE cytoplasm. (B) Comparison of the labeling in the regions apical and basal to the adherens junctions of the RPE (relative amount of label on the melanosome membrane is also indicated). Data were obtained from sections with negligible background labeling (there was no labeling on the adjacent ROSs or extracellular space) and negligible labeling on simultaneously-processed, negative control sections.

View larger version (94K): [in a new window]   Fig. 8. Time-lapse microscopy of melanosome movements in cultured RPE cells. Phase-contrast micrographs of RPE cells cultured from (A) shaker1 control (Myo7a+/4626SB) and (B) shaker1 mutant (Myo7a4626SB/4626SB) mice. Bars, 20 µm. Representative kymograph traces illustrating the movements of individual melanosomes in control (C) and mutant (D) RPE cells. Arrows indicate bursts of rapid movement, and arrowheads indicate periods of slower movement. (E) Histogram illustrating the mean linear displacement resulting from single continuous rapid movements in control and mutant RPE cells (mean±s.d.). (F) Histogram illustrating the percentage of melanosomes per cell moving at different maximal velocities during 350-second periods in control and mutant RPE cells (mean±s.d.).

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