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First published online December 31, 2003
doi: 10.1242/10.1242/jcs.00980

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Forespore membrane assembly in yeast: coordinating SPBs and membrane trafficking

Department of Biology, Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan

View larger version (32K): [in a new window]   Fig. 1. Diagrammatic representation of sporulation stages in the yeasts S. pombe and S. cerevisiae. Stage I, meiotic prophase I. Stage II, meiotic metaphase I/anaphase I. Stage III, meiotic metaphase II/anaphase II; formation of the FSM. Stage IV, completion of FSM assembly. Stage V, spore wall synthesis. Landmark events relevant to the SPB and the FSM are described with reference to cell morphology.

View larger version (119K): [in a new window]   Fig. 2. Electron and fluorescence photomicrographs showing FSM formation near the outer plaque of the SPB in S. pombe. (A and B) Electron microscopy. A blue arrow and a green arrowhead represent SPBs and spindles, respectively. Red asterisks show the FSM. The outer plaques are indicated by a yellow bar. Bar, 0.5 µm. Courtesy of A. Hirata, University of Tokyo, Japan. (C) Fluorescence microscopy. Green, GFP-Psy1 (FSM); Red, Sad1 (SPB); Blue, DNA. Bar, 2 µm. Courtesy of Y. Nakase, Osaka City University, Japan.

View larger version (41K): [in a new window]   Fig. 3. A model for the initiation and development of the FSM on the outer plaque of the SPB. (I) The nucleus contains a three-layered, unmodified SPB. Cytoplasmic vesicles, which carry components of LEPs, are observable. (II) These vesicles gather on the outer plaque of SPBs. Remarkably, the outer plaque develops to be a meiotic outer plaque (MOP), which contains several specific proteins. (III) A FSM precursor is formed by fusion of vesicles. t-SNAREs are recruited to the FSM precursor. A LEP complex is formed at the leading edge of the FSM. (IV) Cells enter anaphase II. The FSM grows by fusion with cytoplasmic vesicles that carry v-SNARE proteins. (V) The FSM engulfs the nucleus and a part of the cytoplasm. Closure of the FSM forms a membrane compartment called the prespore, which contains a haploid nucleus and part of the mother cell cytoplasm.