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First published online 1 March 2005
doi: 10.1242/jcs.01709

Journal of Cell Science 118, 1321-1330 (2005)
Published by The Company of Biologists 2005
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Higher-order nuclear organization in growth arrest of human mammary epithelial cells: a novel role for telomere-associated protein TIN2

Patrick Kaminker1, Cedric Plachot2, Sahn-Ho Kim3, Peter Chung3, Danielle Crippen1, Ole W. Petersen4, Mina J. Bissell3, Judith Campisi1,3 and Sophie A. Lelièvre2,*

1 Buck Institute for Age Research, 8001 Redwood Boulevard, Novato, California 94945, USA
2 Department of Basic Medical Sciences and Cancer Center, Purdue University, 625 Harrison Street, West Lafayette, Indiana 47907-2026, USA
3 Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California, 94720, USA
4 Structural Cell Biology Unit, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen, Denmark.



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  		Fig. 1. Large TIN2 domains are present in HMECs organized into acini. (A) Immunostaining for TIN2 (green) and PNA FISH for telomeres (red) in S1 acini in 3D culture. (B) Immunostaining for TIN2 (green) in a biopsy from normal breast tissue. (C) Dual immunostaining for TIN2 (red) and TRF2 (green) in the nuclei of proliferating 184 HMECs. Colocalization of TIN2 and TRF2 appears yellow. (D) Dual immunostaining for TIN2 (red) and V5 (green) in the nuclei of acini formed by 184 HMECs expressing V5-tagged TIN2 in 3D culture. Nuclei are counterstained with DAPI (blue) in A, C and D, and propidium iodide (red) in B. Images in B and D are confocal sections of acini containing several nuclei. Arrowheads indicate large TIN2 domains. Bar, 5 µm.

 


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  		Fig. 2. Large TIN2 domains are often perinucleolar and colocalized with dense HP 1{gamma} foci. (A) Dual immunostaining for TIN2 (green) and nucleolin (red) in S1 acini in 3D culture. The image is a confocal section of an acinus containing several nuclei, illustrated by the drawing on the left. (B) Dual staining for TIN2 (green) and PML (red) in S1 acini. 3D cultures were untreated (control) or treated for 30 minutes with DNase I (DNase) or RNase A (RNase) prior to immunostaining. (C) Dual immunostaining for HP 1{gamma} (red) and TIN2 (green) in S1 acini. Arrows indicate HP 1{gamma} domains that overlap with large TIN2 domains, arrowheads indicate large TIN2 domains and dashed lines delineate the nuclear periphery. Bar, 5 µm.

 


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  		Fig. 3. Formation of large TIN2 domains in growth-arrested cells is independent of the differentiation status. (A) Large TIN2 domains (arrowheads) revealed by immunostaining (red) in correctly polarized S1 cells cultured in 3D laminin-rich ECM (S1-Matrigel), S1 cells cultured in 3D collagen I (S1-Collagen I) that display altered polarity, and growth-arrested (EGF-deprived) S1 cells cultured as a monolayer on plastic. Nuclei are counterstained with DAPI (blue). (B) Immunostaining for TIN2 (yellow) in growth-arrested 184 cells cultured as a monolayer on plastic. The superimposed phase-contrast image shows the nucleoli as dark gray subnuclear structures. Arrows indicate large TIN2 domains located next to nucleoli. (C) Dual immunostaining for HP 1{gamma} (green) and TIN2 (red) in growth-arrested 184 cells cultured as a monolayer on plastic. Arrowheads indicate overlapping (yellow) HP 1 staining and large TIN2 domains. Nuclei were counterstained with DAPI (blue). Bar, 5 µm.

 


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  		Fig. 4. Formation of large TIN2 domains is independent of telomeres and TRF proteins. (A) HMECs (strain 184) were cultured as a monolayer on plastic and growth-arrested before fixation and dual staining for TIN2 (red) and telomeres (PNA FISH; green) (upper panels), TIN2 (red) and TRF1 (green) (middle panels), and TIN2 (red) and TRF2 (green) (lower panels). (B) Higher magnification images of dual staining for TIN2 (red) and TRF2 (green) showing colocalization at small foci (yellow), indicating telomeric localization. Nuclei were counterstained with DAPI (blue). Arrowheads indicate large TIN2 domains. Bar, 5 µm.

 


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  		Fig. 5. Formation of large TIN2 domains correlates with exit from the cell cycle. (A) Percentage of S1 cells with large TIN2 domain(s) and Ki-67 staining. S1 cells were cultured in 3D for 3 days to obtain a mixed population of cycling Ki-67 positive (Ki-67+) and growth-arrested Ki-67 negative (Ki-67–) cells, and then fixed and dual immunostained for TIN2 and Ki-67. Shown is the percentage of cells containing large TIN2 domains (TIN2 clusters) and Ki-67 positive (filled bars) or negative (open bars) staining. Error bars show the s.e.m. for three different experiments. *P<0.001 when compared with Ki-67+ cells. (B) Dual immunostaining for TIN2 (green) and Ki-67 (red) in S1 cells after 3D culture for 3 days. The different phases of the cell cycle were identified by the pattern of Ki-67 staining. The percentage of cells showing large TIN2 domains (TIN2 clusters) in each phase of the cell cycle is given below each panel, and is the mean±s.e.m. of three different experiments. Arrowheads indicate large TIN2 domains and dashed lines delineate the nuclear periphery. (C) Histogram of the percentage of synchronized 184 HMECs with large TIN2 domains (TIN2 clusters) as a function of the cell cycle. Nuclei showing large TIN2 domains were counted as a percentage of total nuclei (revealed by DAPI counterstaining) during exponential (EXP), G0, S and G2-M phases of the cell cycle. Percentages of Ki-67-positive nuclei in each phase are shown. Bar, 2.5 µm.

 


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  		Fig. 6. TIN2 controls growth arrest in mammary epithelial cells. (A) Schematic of wild-type TIN2 (WT TIN2), and N-terminally (TIN2-13) and C-terminally (TIN2-15) truncated forms of TIN2. (B) Expression of TIN2 and TIN2-13 in control and TIN2-13 expressing cells shown on western blots. Lanes contained cells used for infection (control), cells expressing TIN2-13 (TIN2-13), cells infected with empty vector (control vector), cells overexpressing wild-type TIN2 (TIN2), control HT1080 fibroblasts expressing exogenous TIN2 and TIN2-13 (TIN2 +TIN2-13 mixture in HT1080). Arrows indicate the location of the respective bands for TIN2 and TIN2-13. ß-catenin was used as a loading control. (C) TIN2-15 expression in monolayer and 3D culture shown by anti-myc immunostaining (green). Nuclei were counterstained with DAPI. Magnification x1200. (D) Vector control, TIN2-13 and TIN2-15 infected S1 cells were cultured in 3D for 10 days. Shown are phase-contrast images of acini formed by vector control S1 cells and abnormal looking colonies formed by TIN2-13 and TIN2-15 S1 cells. The arrows indicate enlarged and/or irregular multicellular structures. (E) Non-infected S1 cells (control) and vector control, TIN2-13, or TIN2-15 infected S1 cells were cultured in 3D for 10 days. Acini were classified according to six diameter ranges (6-15 µm, 16-25 µm, 26-35 µm, 36-45 µm, 46-55 µm, 56-65 µm). Shown is the percentage of acini in each diameter range out of a total of 400 acini observed in each independent experiment. Three experiments were performed. (F) Immunostaining for the endogenous basement membrane component collagen IV (red) and Ki-67 (green) in vector control or TIN2-15 S1 cells cultured in 3D for 10 days. When proper morphogenesis occurs, acini are surrounded by a continuous basement membrane and >90% of the cells arrest proliferation. One nucleus positive for Ki-67 is seen out of ten nuclei in vector control; five nuclei positive for Ki-67 are seen out of 14 nuclei in TIN2-15. Arrows indicate the absence of collagen IV around part of the TIN2-15 colony. (G) GFP-S1 cells organized in an acinus (left panel). Immunostaining for the endogenous basement membrane component collagen IV (red) (central panel) and {alpha}6-integrin (green) and ß-catenin (red) (right panel) in hTERT-expressing S1 cells cultured in 3D for 10 days. When proper morphogenesis occurs, in addition to the continuous basement membrane, acini display the localization of {alpha}6-integrin at the basal cell membrane (against the basement membrane) and ß-catenin at cell-cell junctions. (H) Immunostaining for TIN2 (red) in control or TIN2-15 S1 cells. In the control acinus, eight of nine nuclei, identified by DAPI staining, have a large TIN2 domain (arrowheads). In the acinar structure formed by TIN2-15-expressing cells, four of thirteen nuclei show one or two large TIN2 domains (arrowheads) and one nucleus shows completely fragmented TIN2 domains (arrow). Bar, 25 µm.

 

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© The Company of Biologists Ltd 2005