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First published online April 5, 2005
doi: 10.1242/10.1242/jcs.02332

Journal of Cell Science 118, 1559-1563 (2005)
Published by The Company of Biologists 2005
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Multi-lineage potential of fetal cells in maternal tissue: a legacy in reverse

Kiarash Khosrotehrani1 and Diana W. Bianchi2,*

1 Department of Dermatology, Tenon Hospital and UPRES EA2396, Saint-Antoine School of Medicine, Pierre et Marie Curie (Paris VI) University, 75020 Paris, France
2 Division of Genetics, Tufts-New England Medical Center, 750 Washington St, Boston, MA 02111, USA



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  		Fig. 1. Microchimeric fetal cells in female thyroid express cytokeratin. Photomicrographs show FISH studies using Cy3-labeled X (orange) and FITC-labeled Y (green) chromosome probes, and immunofluorescence staining with anti-cytokeratin antibody, Texas Red (red). Nuclei are counterstained with DAPI (blue). (A) Male microchimeric cell in maternal thyroid with one Y chromosome (small arrow) and one X chromosome; 1000x magnification. (B) Same cell stained with anti-cytokeratin antibody (large arrow, red), indicating an epithelial cell; 1000x magnification. (C) Interphase FISH study of female thyroid tissue showing a group of male microchimeric cells; 400x magnification. Arrow shows one X and one Y chromosome. This group of cells did not stain positively for cytokeratin; therefore, they are not epithelial cells. (D) Combined FISH and immunofluorescence staining of a group of male microchimeric cells with one X and one Y (small arrow) chromosome. Note that in this plane of focus not all of the X chromosomes can be seen. This group of cells expresses cytokeratin (large arrow); 400x magnification.

 




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