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First published online November 27, 2006
doi: 10.1242/10.1242/jcs.03269

Journal of Cell Science 119, 4797-4801 (2006)
Published by The Company of Biologists 2006
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Differentiation of columnar epithelia: the hensin pathway

Soundarapandian Vijayakumar1, Jiro Takito1,*, XiaoBo Gao1, George J. Schwartz2 and Qais Al-Awqati1,{ddagger}

1 Departments of Medicine and Physiology and Cellular Biophysics, College of Physicians and Surgeons of Columbia University, 630 W 168th St, New York, NY 10032, USA
2 Department of Pediatrics, University of Rochester School of Medicine, Rochester, NY, USA


Figure 1
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  		Fig. 1. Distribution of cytoskeletal proteins in low-density and high-density cells. X-Z Optical sections of the intercalated cell line cultured at low density, high density and at low density but on the extracellular matrix of high-density cells. The cells were stained by phalloidin and with antibodies to villin and cytokeratin 19. Note the cell height in high-density cells and in cells seeded on high-density matrix.

 

Figure 2
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  		Fig. 2. Domain structure of hensin isoforms.

 

Figure 3
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  		Fig. 3. Development of the mouse embryo between E3 and E4. The upper panels are a schematic of the blastocyst development. A layer of cells (in red) covering the ES cells (orange) becomes the epithelium of the primitive endoderm. Later, some of its cells will populate the inside layer of the trophectoderm to form the parietal endoderm whereas that covering the ES cells will form the visceral endoderm. In the lower panels are embryos taken from mice with LacZ inserted into the hensin locus. The embryo on the right was removed at E3.5 and cultured for 24 hours in vitro. Hensin expression begins in the ES cells (E3.5) and eventually is concentrated in the primitive endoderm.

 

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  		Fig. 4. Epithelial types in the egg cylinder stage. (A) A model of the cell types in the egg cylinder. In the visceral endoderm (VE) only the distal VE and the extra-embryonic VE are columnar epithelia, the rest are flat and squamous looking. The distal VE migrates to establish the anterior VE. As found by Srinivas et al. (Srinivas et al., 2004Go), its shape changes from columnar to squamous during the migration. (B) Expression of hensin (in blue) in the egg cylinder stage. Note that the embryo lacks parietal endoderm.

 

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© The Company of Biologists Ltd 2006