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First published online March 22, 2006
doi: 10.1242/10.1242/jcs.02802

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KGF suppresses ₂ß₁ integrin function and promotes differentiation of the transient amplifying population in human prostatic epithelium

¹ Urology Research Group, Northern Institute for Cancer Research, University of Newcastle, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK
² YCR Cancer Research Unit (Area Department of Biology, University of York, PO Box 373, York, YO10 5DD, UK
³ FACS laboratory, Surgical and Reproductive Sciences, University of Newcastle, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK

View larger version (25K): [in a new window]   Fig. 1. Methylcellulose (MC) suspension induces differentiation. (A) Control 2ß1hi and MC-treated 2ß1hi prostatic epithelial cells stained for PAP (FITC/green) and the nuclear stain DAPI (blue). Inset is at increased magnification (x800). (B) Bar chart summarising PAP expression in 2ß1hi cells. Data are mean ± s.e.m. of three experiments.

View larger version (22K): [in a new window]   Fig. 2. Blocking ß1 integrin function induces differentiation. (A) Dot plots of PAP expression following blocking with anti-ß1-integrin antibody. Controls were set at 3%. (B) Bar chart summarising the effect of blocking ß1 integrin on the expression of differentiation markers. Data are mean ± s.e.m. of three experiments. (C) Expression of differentiation markers CK18, PAP and AR (FITC/green) in 2ß1hi cells treated with ß1-blocking antibody. Cells were counterstained with the nuclear stain DAPI (blue).

View larger version (82K): [in a new window]   Fig. 3. Changes in cell morphology and cell cycle with blockade of the integrin ß1. (A) Scanning electron microscopy of 2ß1hi cells treated with the ß1-blocking antibody. (B) Effect on the cell cycle following 2ß1hi cell treatment with ß1-blocking antibody. Data are mean ± s.e.m. of three experiments. Bars, 20 µm.

View larger version (15K): [in a new window]   Fig. 4. Relationship between the dose of ß1-blocking antibody and 2ß1hi cell differentiation and adhesion. Effect of the ß1-blocking IgG on the number of cells adhering to collagen type-1 and expression of the differentiation marker PAP. Data are mean ± s.e.m. of three experiments.

View larger version (25K): [in a new window]   Fig. 5. Effect of KGF treatment on 2ß1hi cells. (A) Bar chart summarising the effect of KGF on expression of differentiation markers PAP, CK18 and AR (measured by FACS) in 2ß1hi cells. (B) Triple immunofluorescence study of 2ß1hi cells treated with KGF. AR, TRITC/red; PSA, FITC/green; DAPI, blue. Colocalisation is indicated in yellow. (C) RT-PCR for KGFR mRNA expression in primary prostate cell sub-populations. *PEC, primary culture of epithelial cells following subtraction of 2ß1hi basal cells. ß-actin levels were detected as a control (lower gel). (D) Summary of FACS analysis of AR expression in 2ß1hi cells untreated or treated with combinations of KGF and the ß1-blocking antibody. Data are mean ± s.e.m. of three experiments.

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