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First published online June 4, 2008
doi: 10.1242/10.1242/jcs.023630

Journal of Cell Science 121, 1955-1963 (2008)
Published by The Company of Biologists 2008
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The multiple roles of PtdIns(4)P – not just the precursor of PtdIns(4,5)P2

Giovanni D'Angelo, Mariella Vicinanza, Antonella Di Campli and Maria Antonietta De Matteis*

Laboratory of Secretion Physiopathology, Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro (CH), Italy


Figure 1
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  		Fig. 1. The metabolic cycle of PtdIns(4)P in yeast and mammals. The metabolic cycle of PtdIns(4)P in yeast and mammals. PtdIns(4)P can be produced through phosphorylation of PtdIns by PI4Ks and through dephosphorylation of PtdIns(4,5)P2 by PtdIns(4,5)P2-5-phosphatases (5-phosphatases). These comprise Sjl1p, Sjl2p and Sjl3p in yeast, and several members in mammals, including synaptojanin 1 and synaptojanin 2, OCRL, INPP5B, INPP5E, INPP5F, PAB5PA and SKIP. Enzymes are indicated by ellipses and the regulatory factors are connected to the PI4Ks either by continuous lines (physical interactions) or by dashed lines (functional interactions). See text for details.

 

Figure 2
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  		Fig. 2. Intracellular distribution of PI4Ks and PtdIns(4)P effectors in mammalian cells. The intracellular localisation of PI4Ks and PtdIns(4)P effectors in mammalian cells suggests a central role for the Golgi complex in the synthesis and biological function of PtdIns(4)P. N, nucleus; GC, Golgi complex.

 

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  		Fig. 3. PtdIns(4)P effectors. (A,B) Domain organisation of mammalian and yeast proteins that interact with PtdIns(4)P. PtdIns(4)P binding sites are indicated by a star. VHS-GGA; (Vps27p/Hrs/STAM-GGA domain); ANK-REP; ankyrin repeats; SH3; Src-homology domain 3; PB1; Phox and Bem1p domain.

 

Figure 4
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  		Fig. 4. Golgi localisation of the PH domain of FAPP1 and OSBP1. COS-7 cells expressing the GFP-tagged PH domain of OSBP1 (OSBP-PH) and MDCK cells expressing the GFP-tagged PH domain of FAPP1 (FAPP1-PH) were labelled for the Golgi marker GM130 (red) and processed for immunofluorescence. MDCK cells expressing the GST-tagged FAPP1-PH were processed for immuno-electron microscopy and labelled with anti-GST antibodies (far right panel). Notice the polarised distribution of FAPP1-PH at the trans-pole of the Golgi stacks, as indicated by the presence of a clathrin-coated profile (arrowhead). See text for details.

 

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© The Company of Biologists Ltd 2008