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First published online December 31, 2008
doi: 10.1242/10.1242/jcs.035246

Journal of Cell Science 122, 227-232 (2009)
Published by The Company of Biologists 2009
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Mice that lack activity of {alpha}vβ6- and {alpha}vβ8-integrins reproduce the abnormalities of Tgfb1- and Tgfb3-null mice

Poshala Aluwihare1, Zhenyu Mu1, Zhicheng Zhao1, Dawen Yu1, Paul H. Weinreb2, Gerald S. Horan2, Shelia M. Violette2 and John S. Munger1,*

1 Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
2 Biogen Idec, Cambridge, MA 02142, USA


Figure 1
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  		Fig. 1. Phenotype of adult Itgb8–/– mice. (A) Itgb8–/– mice are smaller than control mice. White bars show control weights (n=13), black bars show Itgb8–/– weights (n=14). Error bars represent the s.e.m. (B) Representative hematoxylin- and eosin-stained sections of liver and colon of Itgb8–/– mice (9 weeks of age, original magnification 400x) demonstrate lack of inflammation. Scale bar: 100 µm.

 

Figure 2
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  		Fig. 2. Cleft palate in Itgb6–/–;Itgb8–/– neonates. The control sample shows normally fused palatal shelves, whereas the Itgb6–/–;Itgb8–/– sample demonstrates a cleft of the secondary palate. Arrows indicate cleft palate.

 

Figure 3
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  		Fig. 3. (A) Kaplan-Meier survival curves for Itgb8–/– mice (n=17), Itgb8–/– mice with 6.3G9 treatment starting at P3 (n=4), Itgb8–/– mice with 6.3G9 treatment starting at P0 (n=8), and Itgb8–/– mice with 6.3G9 treatment starting at E16.5 (n=12). (B) Hematoxylin and eosin staining of liver, lung, stomach and heart of representative Itgb8–/– and littermate non-KO control mice treated with 6.3G9 starting at E16.5 and sacrificed at 19 days. (C) Itgb8–/– mice treated with 6.3G9 starting at E16.5 lack Langerhans cells (LCs). Representative examples of epidermal sheets stained with an antibody that recognizes LCs. (D) Tregs isolated from thymus and spleen were identified by staining for FOXP3. Cells are from control mice, Itgb8–/– mice, and control and Itgb8–/– mice treated with 6.3G9 starting at E16.5. (n=3-4 for thymus, n=3 for spleen). Error bars represent the s.e.m. Scale bar: 100 µm.

 

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© The Company of Biologists Ltd 2009