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First published online 24 February 2009
doi: 10.1242/jcs.041731

Journal of Cell Science 122, 849-858 (2009)
Published by The Company of Biologists 2009
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A novel role for SED1 (MFG-E8) in maintaining the integrity of the epididymal epithelium

Adam S. Raymond and Barry D. Shur*

Department of Cell Biology, Biochemistry, Cell and Developmental Biology Graduate Program, Emory University School of Medicine, Atlanta, GA 30322, USA


Figure 1
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  		Fig. 1. Epididymides from SED1-null males show epithelial breakdown. (A) Photomicrographs of paraffin-embedded epididymal tissue with the major functional regions identified. Sagittal sections from SED1 heterozygous (+/–) and null (–/–) littermates at 8 weeks of age stained with hematoxylin and eosin and imaged at identical magnification. The caudal segment of the SED1-null (–/–) epididymis contains a large spermatic granuloma (arrow) characterized by breakdown of the tubule and resulting in a bolus of sperm, fluid and immune cells. Occlusion of the tubule results in severe tubule swelling upstream of the lesion (compare corpus regions) and an absence of sperm in downstream tubules (arrowhead). (B-E) Other SED1-null pathologies not directly associated with the lesion site include detached, free-floating cells within the lumen suggestive of shed epithelium (arrowheads, B,D,E). (C) is an enlargement of (B). Scale bars: 50 µm (B), 20 µm (C-E).

 

Figure 2
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  		Fig. 2. SED1 localizes to both apical and basolateral domains of epididymal epithelial cells in vivo. (A) Composite photomicrograph of an epididymal sagittal-section positioned adjacent to a size-appropriate schematic of the testis. The initial segment, caput, corpus and cauda regions are identified. (B) Immunoblotting for SED1 in three epididymal regions; samples are loaded with equal protein. Immunoblotting of wild-type (+/+) tissue results in two bands consistent with the small and large isoforms of SED1. SED1-null (–/–) tissue produces background immunoreactivity. β-tubulin (tub) serves as a loading control. (C) In the initial segment, SED1 is found in punctate and often filamentous arrays along basolateral borders between adjacent epithelial cells (tissue perfused in 4% PFA). SED1 is also found in vesicles associated with the basal domain of nonprincipal cells, known as clear cells, in the caput, corpus and cauda segments (tissue submersion-fixed in Bouin's). SED1-null (–/–) tissue shows only background immunoreactivity. (D) Double-label immunofluorescence with E-cadherin (green) illustrates that many of the SED1 plagues (red) seen in the initial segment lie on the cell border (arrowheads). Two examples are shown. The base of the epithelium is designated by small arrows. (E,F) SED1-positive (red) clear cells of the caput and corpus regions fail to stain for the lysosomal marker, LAMP-1 (green), although clear cells in more distal regions (i.e. caput), are LAMP-1 positive, suggesting the protein is not destined for degradation in the proximal segments (caput, corpus) as it appears to be in the distal cauda. Images in panel C represent a merged stack of confocal Z-sections taken through the tissue, whereas those in panels D-F are merged epifluorescence photomicrographs. L, lumen; N, nucleus. Scale bars: 20 µm (C); 10 µm (D-F).

 

Figure 3
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  		Fig. 3. Polarized primary epididymal epithelial cells secrete SED1 both apically and basally. (A,A') Immunofluorescence of the apical zonal occludin protein ZO-1 (green) and basal nuclei (red) in primary epididymal epithelial cells grown on Matrigel-coated transwell filters. X-Y projections and companion z-plane cross-sections (black arrow; white line indicates cross-section location) generated by confocal z-stack imaging show wild-type and SED1-null primary cells polarize and form apical tight junctions in culture. The cell diagram illustrates the approximate depth of confocal scans taken of polarized cultures immunostained with SED1 antisera. (B) In polarized cultures of wild-type cells, SED1 appears as punctate bodies in the apical domain of the cell consistent with its known apical secretion. (C) Little SED1 protein is found at the level of the nuclei; however immunoreactivity returns in the sub-nuclear basal domain (D). Examination reveals this signal is found in the same z-plane as the filter suggesting cells deposit SED1 onto the underlying substrate. (B',C',D') SED1-null cultures assayed in parallel have no immunoreactivity. Scale bars: 20 µm.

 

Figure 4
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  		Fig. 4. Exogenous SED1 supports epididymal epithelial cell adhesion in a dose-dependent manner. (A) Increasing levels of SED1 lead to a concomitant increase in cell adhesion, reaching maximal values at 250 ng/well. Cells do not adhere to uncoated `mock' wells. (B) Representative photomicrographs of crystal violet-stained adherent cells on SED1 substrates. (C) Cytokeratin immunoreactivity confirms that the adherent cells are predominately epithelial. red, cytokeratin; green, nuclei (SYTO24). Error bars: s.d.

 

Figure 5
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  		Fig. 5. The addition of RGD peptides is sufficient to block the initial adhesion of epididymal epithelial cells to SED1. Cells preincubated with medium supplemented with 100 µM RGD or control (RAD) peptides were added to 30-minute adhesion assays on substrates coated with 500 ng/well SED1 or laminin. Data are expressed as a percentage of untreated (mock) cell adhesion to each substrate. RGD reduces adhesion to SED1 by 92% but has no effect on adhesion to laminin. RAD has a mild nonspecific effect on cell adhesion to both substrates. Error bars: s.e.m. *P<0.0001.

 

Figure 6
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  		Fig. 6. The {alpha}V integrin subunit is expressed in epididymal epithelial cells and localizes to focal plaques in cells adherent to SED1. (A) Immunoblotting for {alpha}V in the three epididymal regions; samples are loaded with equal protein. Immunoblotting of wild-type (+/+) and SED1-null (–/–) tissue under nonreducing conditions results in a single prominent band at 125 kDa, the reported molecular weight for {alpha}V under nonreduced conditions. Wild-type and SED1-null tissue express {alpha}V at similar levels. (B) Similarly, lysates of epithelial-enriched primary epididymal cells immunoprobed for {alpha}V exhibit a single 125 kDa band. β-tubulin serves as a loading control. (C) Immunostaining of cells cultured on SED1 reveals cytokeratin-positive epididymal epithelial cells (red) that express {alpha}V integrins (green) in punctate bundles arranged along the basal surface. (D) Desmin-positive (red) smooth muscle cells exhibit little or no {alpha}V immunoreactivity. Nonimmune (NI) stained cells produce background immunoreactivity. All images are from parallel experiments imaged under identical conditions. (E) Confocal micrographs reveal that cytokeratin-positive (inserts) epithelial cells cultured on SED1 localize {alpha}V to focal plaques along the lamellipodia (arrows). (F) Epithelial cells cultured on laminin also express {alpha}V; however, the immunoreactivity is not distributed in focal plaques as on SED1 substrates but remains perinuclear. Scale bars: 20 µm.

 

Figure 7
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  		Fig. 7. A small molecule inhibitor of {alpha}Vβ3 and {alpha}Vβ5 integrin heterodimers (L-954) selectively blocks epididymal cell adhesion to SED1. Increasing concentrations of L-594 were added to 30-minute adhesion assays, eliciting a dose-dependent decrease in adhesion to SED1 substrates (500 ng/well). The inhibitor has little effect on cell adhesion to laminin-coated substrates (500 ng/well). Data are expressed as a percentage of untreated (mock) cell adhesion to each substrate. As little as 1 nM of inhibitor is sufficient to reduce adhesion to SED1 by 19%, while 1000 nM inhibits adhesion by 93%. Error bars: s.e.m. *P<0.0001.

 

Figure 8
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  		Fig. 8. Function-blocking antibodies against {alpha}V integrin block epididymal cell adhesion to SED1. The addition of {alpha}V-blocking IgG (RMV-7) (50 µg/ml or 100 µg/ml) results in a dose-dependent reduction in epithelial cell adhesion to SED1 of 18% and 57%, respectively. Control IgG has no effect. Data are expressed as a percentage of untreated (mock) cell adhesion. Error bars: s.e.m. *P<0.0001.

 

Figure 9
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  		Fig. 9. Loss of SED1 leads to deficient cellular adhesion in vitro. Primary epithelial-enriched epididymal cells were isolated and cultured for 4 days on uncoated glass or SED1 substrates. Attached cells were pulsed with BrdU for 4 hours, fixed, and immunostained for BrdU. Epididymal `islands' (arrowheads) containing ≥4 nuclei were counted. (A) Epifluorescent imaging of SYTO24 counterstain illustrates a reduction in the number of SED1-null (–/–) epithelial islands relative to wild type (+/+). This phenotype is rescued when cells are cultured on SED1 substrates; under these conditions cells of both genotypes produce abundant islands. (B) Quantification shows that SED1-null (–/–) cells generate 35% fewer islands than wild-type (+/+) cells when cultured on glass substrates. (C) BrdU-positive nuclei associated with epithelial islands were counted and expressed as a percentage of total nuclei associated with islands. Data from multiple experiments were averaged and the wild-type (+/+) rate of proliferation was normalized to 100%. Although SED1-null (–/–) cells generate fewer islands than wild type, the rate of proliferation for the two genotypes is identical on glass substrates. Furthermore, the presence of SED1 substrates does not lead to increased proliferation relative to cells cultured on glass. Scale bar: 100 µm. Error bars: s.e.m. *P=0.01; **P<0.001.

 

Figure 10
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  		Fig. 10. Working model of SED1-dependent epididymal epithelial cell adhesion. The EFG domains (orange circles) and discoidin domains (green ovals) of SED1 are depicted bound to epididymal epithelial cells. The second EGF domain of SED1 contains a RGD motif and is proposed to serve as ligand for {alpha}Vβ3 or {alpha}Vβ5 integrins (blue receptor) expressed by the epithelial cells. Two models of adhesion are presented: (A) cell-cell adhesion and (B) cell-basement membrane adhesion. The discoidin domains are predicted to function by binding either (A) anionic phospholipids on the cell surface or (B) negatively charged residues within the basement membrane.

 

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© The Company of Biologists Ltd 2009