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Association of cortactin with dynamic actin in lamellipodia and on endosomal vesicles

Marko Kaksonen¹, H. Benjamin Peng² and Heikki Rauvala¹

¹Laboratory of Molecular Neurobiology, Department of Biosciences and Institute of Biotechnology, PO Box 56, FIN-00014, University of Helsinki, Finland

²Department of Cell Biology and Anatomy, Neuroscience Center and Curriculum in Neurobiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599, USA

Dynamic behavior of cortactin labeled structures

A time-lapse series of a cortactin-RFP expressing cell. Cell center is on the left and a cortactin-RFP labeled lamellipodium is on the right. Time-lapse series reveals the retrograde movement of cortactin in the lamellipodium and the movement of the cytoplasmic spots. Total acquisition time was 5 minutes and interval between each image is 6 seconds. Image field is 2717 µm.

Visualization of cortactin and actin in living cells

A cell expressing cortactin-RFP (red, left frame) and GFP-actin (green, middle frame) was imaged for 4 minutes. Overlay of the two channels is shown on the right. Similar retrograde movement in the lamellipodium is seen with both fusion proteins. Stable actin structures do not contain cortactin. Image fields are 2319 µm.

Movement of vesicles with cortactin-GFP tails

Time-lapse imaging suggests that cortactin-GFP labeled tails propel vesicle movement. Total acquisition time was 4 minutes and interval between each image is 6 seconds. Image field is 4.67.5 µm.

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