QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Summary Services Email this article to a friend Alert me to new issues of the journal

Accumulation of profilin II at the surface of Listeria is concomitant with the onset of motility and correlates with bacterial speed

Marcus Geese, Kathrin Schlüter, Martin Rothkegel, Brigitte M. Jockusch, Jürgen Wehland and Antonio S. Sechi

Department of Cell Biology, Gesellschaft für Biotechnologische Forschung (GBF), Mascheroder Weg 1, D-38124 Braunschweig, Germany

Movie Fig. 3

Dynamics of profilin II-GFP in B16-F1 cells. Video microscopy frames showing the distribution of profilin II-GFP (upper panel) during the spreading of a B16-F1 cell and corresponding phase contrast images (lower panel). Profilin II-GFP predominately localises as a thin line at the front of a spreading lamellipodium. Point-like structures showing lateral movement could be observed (arrowhead).

Movie Fig. 4

Profilin II-GFP associates with Listeria at the onset of motility. PtK2 cells expressing GFP-tagged profilin II were infected with Listeria monocytogenes wild type and observed by time-lapse videomicroscopy. Left panel represents profilin II-GFP images, whereas right panel shows the corresponding phase contrast images. Profilin II-GFP localises to motile bacteria (arrowheads 1 and 2) but it is not detectable on stationary ones (arrowheads 3 and 4).

Movie Fig. 5

Correlation between accumulation of profilin II-GFP and bacterial movement. PtK2 cells were transfected with profilin II-GFP and then infected with Listeria monocytogenes. Progressive accumulation of profilin II-GFP (upper panel) at the rear pole of a bacterium (arrowhead) can be observed in correspondence of onset of actin-driven Listeria motility. Conversely, a decrease of profilin II-GFP associated with the same bacterium corresponded to the arrest of the bacterial motility (arrowhead). Lower panel shows the corresponding phase contrast images.

Movie Fig. 6

Profilin II-GFP does not associate with Listeria monocytogenes [delta]ActA5. PtK2 cells transfected with profilin II-GFP were infected with a Listeria mutant which expressed a mutated form of the ActA protein lacking the central proline-rich region. The lower panel shows phase contrast images, whereas the upper panel shows the corresponding fluorescence micrographs. In contrast with the situation typical of the wild type Listeria, profilin II-GFP does not associate either with motile bacteria or with stationary ones.

This Article Summary Services Email this article to a friend Alert me to new issues of the journal