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Large-scale chromatin organization of the major histocompatibility complex and other regions of human chromosome 6 and its response to interferon in interphase nuclei

Emanuela V. Volpi, Edith Chevret, Tania Jones, Radost Vatcheva, Jill Williamson, Stephan Beck, R. Duncan Campbell, Michelle Goldsworthy, Stephen H. Powis, Jiannis Ragoussis, John Trowsdale and Denise Sheer

Human Cytogenetics Laboratory, Imperial Cancer Research Fund, London WC2A 3PX, UK

Movie1

A fibroblast nucleus hybridized with a chromosome 6 short arm specific paint (red) and a pool of 10 probes covering 700 kb of the MHC class III region (green), corresponding to Figure 3d, Volpi, Chevret et al. The nucleus itself is unstained. The MHC class III region appears external to the painted domain of one chromosome 6 homologue and at the periphery of the painted domain of the other chromosome 6 homologue. Optical sections were obtained by confocal microscopy and three-dimensional reconstruction performed using Voxel View Ultra 2.1.2 software on a Silicon Graphics Workstation.

Movie2

B-lymphoblastoid cell nucleus hybridized with a chromosome 6 short arm specific paint (green) and a pool of 15 probes covering 850 kb of the MHC class II region (red), from Volpi, Chevret et al. The nucleus itself is unstained. The MHC class II region appears at the periphery of the painted domain of each chromosome 6 homologue. Optical sections were obtained by confocal microscopy and three-dimensional reconstruction performed using Voxel View Ultra 2.1.2 software on a Silicon Graphics Workstation.

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