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Recruitment and activation of Rac1 by the formation of E-cadherin-mediated cell-cell adhesion sites

Masato Nakagawa¹, Masaki Fukata², Masaki Yamaga¹, Naohiro Itoh¹ and Kozo Kaibuchi^1,2

¹Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630-0101, Japan
²Department of Cell Pharmacology, Nagoya University, Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya, 466-8550, Japan

In MDCKII cells transfected with pEGFP-Rac1 wild-type and cultured in normal Ca2+ levels (~1.8 mM), EGFP-Rac1 was localized at sites of cell-cell contact. Whereas, in cells treated with EGTA, EGFP-Rac1 was translocated from sites of cell-cell contact to the cytosol and became diffusely distributed there. After the restoration of Ca2+, EGFP-Rac1 was detected at sites of cell-cell contact. The formation of E-cadherin-mediated cell-cell adhesion sites was completed 120 minutes after the restoration.

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