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Fig. 4. Hepatocyte gap junctions remain open during InsP3- dependant agonists stimulation. Typical calcein fluorescence recovery analysis. Each curve was normalized and then fitted to an exponential function (red line) as described in Materials and Methods. Analysis allowed us to obtain two values: the degree of recovery (%) and the rate of recovery ({tau}). As shown here, recovery was large (>60%) and rapid ({tau}{approx}100 seconds) in the control experiment. By contrast, octanol reduced almost totally the fluorescence recovery after photobleach.





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