Fig. 11. The DNA polymerase 
complexed with Dpit47 is inactive. DNA polymerase was immunoprecipitated using anti-Dpit47 antibody or monoclonal anti-DNA polymerase antibody. Top panel, DNA polymerase assay: immunoprecipitated DNA polymerase was used to carry out an end filling polymerase assay on a 35 mer as described in Materials and Methods. The reaction was stopped after 5, 15 or 30 minutes. The product of the reaction was analysed on a 15% polyacrylamide gel. Lower panel shows by western blotting the amount of DNA polymerase contained in the Dpit47 (Dpit47 IP) and DNA polymerase (pol IP) immunoprecipitates used in the assay.
