Fig. 2. Double immunocytochemical staining of an intact monolayer of HRA-19 cells. (A) Chromogranin-A-positive endocrine cells (arrows). (B) Type-IV-collagen staining in the same cells as (A) (arrows). Notice that the staining is restricted to individual cells. Faint staining over the whole monolayer is non-specific background staining, indistinguishable from the no-primary-antibody controls. Bar, 50 µm.

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