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Fig. 4. Time-course analysis of endocrine- and goblet-lineage commitment in HRA-19 cells following the transfer of cells at day 0 to serum-free medium. Total number of cells expressing prolyl-4-hydroxylase and collagen IV in each replicate monolayer can be seen in relation to the numbers of endocrine (chromogranin-A positive) and goblet (colonic-mucus positive) cells.





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